Acrylamide in polyacrylamide gels can modify proteins during electrophoresis.
Publication
, Journal Article
Bonaventura, C; Bonaventura, J; Stevens, R; Millington, D
Published in: Anal Biochem
October 1994
Many notable discoveries have resulted from the characterization or purification of compounds by electrophoresis. The results reported here show that when polyacrylamide gels are used as the support matrix for electrophoresis, proteins can be modified by reaction with unpolymerized monomers of acrylamide, forming covalent acrylamide adduction products. This was demonstrated by electrophoresis of human and rat hemoglobins and subsequent analysis by electrospray ionization mass spectrometry. The degree of covalent acrylamide binding can be appreciable. The degree of adduction is dependent upon the condition of the gel matrix, the amount of material applied, and the reactivity of the material under investigation.
Duke Scholars
Published In
Anal Biochem
DOI
ISSN
0003-2697
Publication Date
October 1994
Volume
222
Issue
1
Start / End Page
44 / 48
Location
United States
Related Subject Headings
- Rats, Sprague-Dawley
- Rats
- Protein Binding
- Polymers
- Kinetics
- Humans
- Hemoglobins
- Electrophoresis, Polyacrylamide Gel
- Biochemistry & Molecular Biology
- Animals
Citation
APA
Chicago
ICMJE
MLA
NLM
Bonaventura, C., Bonaventura, J., Stevens, R., & Millington, D. (1994). Acrylamide in polyacrylamide gels can modify proteins during electrophoresis. Anal Biochem, 222(1), 44–48. https://doi.org/10.1006/abio.1994.1451
Bonaventura, C., J. Bonaventura, R. Stevens, and D. Millington. “Acrylamide in polyacrylamide gels can modify proteins during electrophoresis.” Anal Biochem 222, no. 1 (October 1994): 44–48. https://doi.org/10.1006/abio.1994.1451.
Bonaventura C, Bonaventura J, Stevens R, Millington D. Acrylamide in polyacrylamide gels can modify proteins during electrophoresis. Anal Biochem. 1994 Oct;222(1):44–8.
Bonaventura, C., et al. “Acrylamide in polyacrylamide gels can modify proteins during electrophoresis.” Anal Biochem, vol. 222, no. 1, Oct. 1994, pp. 44–48. Pubmed, doi:10.1006/abio.1994.1451.
Bonaventura C, Bonaventura J, Stevens R, Millington D. Acrylamide in polyacrylamide gels can modify proteins during electrophoresis. Anal Biochem. 1994 Oct;222(1):44–48.
Published In
Anal Biochem
DOI
ISSN
0003-2697
Publication Date
October 1994
Volume
222
Issue
1
Start / End Page
44 / 48
Location
United States
Related Subject Headings
- Rats, Sprague-Dawley
- Rats
- Protein Binding
- Polymers
- Kinetics
- Humans
- Hemoglobins
- Electrophoresis, Polyacrylamide Gel
- Biochemistry & Molecular Biology
- Animals