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Cytochrome c oxidase binding of hydrogen peroxide.

Publication ,  Journal Article
Bickar, D; Bonaventura, J; Bonaventura, C
Published in: Biochemistry
May 1982

Oxidized cytochrome c oxidase can bind hydrogen peroxide, as evidenced by changes in its spectrum and its ability to use hydrogen peroxide as an electron acceptor in cytochrome c oxidation. The affinity of the oxidized enzyme for hydrogen peroxide is high, with a Kd of less than 10 microM, and the binding is inhibited by ligands of cytochrome a3. Oxidized cytochrome c oxidase, in submitochondrial particles or solubilized in several ionic and nonionic detergents, binds peroxide with comparable affinities. The size of the spectral shift observed upon peroxide binding depends on the pH of the solution and differs in extinction coefficient between preparations, but all preparations tested appeared to bind peroxide. The differences in the magnitude of the spectral shift upon peroxide binding to different preparations suggest that oxidized cytochrome c oxidase as prepared may be made up of more than one species and that the proportion of the species which binds peroxide varies with the preparation. These studies of the binding of peroxide clarify the mechanism by which cytochrome c oxidase catalyzes the reduction of oxygen to water without the formation of free-radical intermediates.

Duke Scholars

Published In

Biochemistry

DOI

EISSN

1520-4995

ISSN

0006-2960

Publication Date

May 1982

Volume

21

Issue

11

Start / End Page

2661 / 2666

Related Subject Headings

  • Time Factors
  • Kinetics
  • Hydrogen-Ion Concentration
  • Hydrogen Peroxide
  • Electron Transport Complex IV
  • Cytochrome c Group
  • Biochemistry & Molecular Biology
  • 3404 Medicinal and biomolecular chemistry
  • 3205 Medical biochemistry and metabolomics
  • 3101 Biochemistry and cell biology
 

Citation

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MLA
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Bickar, D., Bonaventura, J., & Bonaventura, C. (1982). Cytochrome c oxidase binding of hydrogen peroxide. Biochemistry, 21(11), 2661–2666. https://doi.org/10.1021/bi00540a013
Bickar, D., J. Bonaventura, and C. Bonaventura. “Cytochrome c oxidase binding of hydrogen peroxide.Biochemistry 21, no. 11 (May 1982): 2661–66. https://doi.org/10.1021/bi00540a013.
Bickar D, Bonaventura J, Bonaventura C. Cytochrome c oxidase binding of hydrogen peroxide. Biochemistry. 1982 May;21(11):2661–6.
Bickar, D., et al. “Cytochrome c oxidase binding of hydrogen peroxide.Biochemistry, vol. 21, no. 11, May 1982, pp. 2661–66. Epmc, doi:10.1021/bi00540a013.
Bickar D, Bonaventura J, Bonaventura C. Cytochrome c oxidase binding of hydrogen peroxide. Biochemistry. 1982 May;21(11):2661–2666.
Journal cover image

Published In

Biochemistry

DOI

EISSN

1520-4995

ISSN

0006-2960

Publication Date

May 1982

Volume

21

Issue

11

Start / End Page

2661 / 2666

Related Subject Headings

  • Time Factors
  • Kinetics
  • Hydrogen-Ion Concentration
  • Hydrogen Peroxide
  • Electron Transport Complex IV
  • Cytochrome c Group
  • Biochemistry & Molecular Biology
  • 3404 Medicinal and biomolecular chemistry
  • 3205 Medical biochemistry and metabolomics
  • 3101 Biochemistry and cell biology