Functional properties of normal and sickle cell hemoglobins in polyethylene glycol 6000.
Publication
, Journal Article
Amiconi, G; Bonaventura, C; Bonaventura, J; Antonini, E
Published in: Biochimica et biophysica acta
December 1977
The functional properties of human hemoglobin A and S were studied in concentrated solutions of polyethylene glycol. Polyethylene glycol solutions are frequently used as media for protein crystallization. In particular, sickle cell hemoglobin, which does not make X-ray quality crystals in high salt solutions, will form high-quality crystals in polyethylene glycol. Comparison of the functional properties of normal and sickle cell hemoglobin in polyethylene glycol show that pH, anion effects and cooperativity of ligand binding are largely unaffected by polyethylene glycol. This suggests that the crystals grown in this medium are representative of the native structure.
Duke Scholars
Published In
Biochimica et biophysica acta
DOI
EISSN
1878-2434
ISSN
0006-3002
Publication Date
December 1977
Volume
495
Issue
2
Start / End Page
279 / 286
Related Subject Headings
- Polyethylene Glycols
- Phytic Acid
- Oxyhemoglobins
- Kinetics
- Hydrogen-Ion Concentration
- Humans
- Hemoglobins
- Hemoglobin, Sickle
- Diphosphoglyceric Acids
- 51 Physical sciences
Citation
APA
Chicago
ICMJE
MLA
NLM
Amiconi, G., Bonaventura, C., Bonaventura, J., & Antonini, E. (1977). Functional properties of normal and sickle cell hemoglobins in polyethylene glycol 6000. Biochimica et Biophysica Acta, 495(2), 279–286. https://doi.org/10.1016/0005-2795(77)90384-1
Amiconi, G., C. Bonaventura, J. Bonaventura, and E. Antonini. “Functional properties of normal and sickle cell hemoglobins in polyethylene glycol 6000.” Biochimica et Biophysica Acta 495, no. 2 (December 1977): 279–86. https://doi.org/10.1016/0005-2795(77)90384-1.
Amiconi G, Bonaventura C, Bonaventura J, Antonini E. Functional properties of normal and sickle cell hemoglobins in polyethylene glycol 6000. Biochimica et biophysica acta. 1977 Dec;495(2):279–86.
Amiconi, G., et al. “Functional properties of normal and sickle cell hemoglobins in polyethylene glycol 6000.” Biochimica et Biophysica Acta, vol. 495, no. 2, Dec. 1977, pp. 279–86. Epmc, doi:10.1016/0005-2795(77)90384-1.
Amiconi G, Bonaventura C, Bonaventura J, Antonini E. Functional properties of normal and sickle cell hemoglobins in polyethylene glycol 6000. Biochimica et biophysica acta. 1977 Dec;495(2):279–286.
Published In
Biochimica et biophysica acta
DOI
EISSN
1878-2434
ISSN
0006-3002
Publication Date
December 1977
Volume
495
Issue
2
Start / End Page
279 / 286
Related Subject Headings
- Polyethylene Glycols
- Phytic Acid
- Oxyhemoglobins
- Kinetics
- Hydrogen-Ion Concentration
- Humans
- Hemoglobins
- Hemoglobin, Sickle
- Diphosphoglyceric Acids
- 51 Physical sciences