Functional properties of normal and sickle cell hemoglobins in polyethylene glycol 6000.

Journal Article

The functional properties of human hemoglobin A and S were studied in concentrated solutions of polyethylene glycol. Polyethylene glycol solutions are frequently used as media for protein crystallization. In particular, sickle cell hemoglobin, which does not make X-ray quality crystals in high salt solutions, will form high-quality crystals in polyethylene glycol. Comparison of the functional properties of normal and sickle cell hemoglobin in polyethylene glycol show that pH, anion effects and cooperativity of ligand binding are largely unaffected by polyethylene glycol. This suggests that the crystals grown in this medium are representative of the native structure.

Full Text

Duke Authors

Cited Authors

  • Amiconi, G; Bonaventura, C; Bonaventura, J; Antonini, E

Published Date

  • December 20, 1977

Published In

Volume / Issue

  • 495 / 2

Start / End Page

  • 279 - 286

PubMed ID

  • 22353

International Standard Serial Number (ISSN)

  • 0006-3002

Language

  • eng

Conference Location

  • Netherlands