Allylation of intraerythrocytic hemoglobin by raw garlic extracts.

Journal Article (Journal Article)

Recent studies have shown that deoxygenated human red blood cells (RBCs) converted garlic-derived polysulfides into hydrogen sulfide, which in turn produced vasorelaxation in aortic ring preparations. The vasoactivity was proposed to occur via glucose- and thiol-dependent acellular reactions. In the present study, we investigated the interaction of garlic extracts with human deoxygenated RBCs and its effect on intracellular hemoglobin molecules. The results showed that garlic extract covalently modified intraerythrocytic deoxygenated hemoglobin. The modification identified consisted of an addition of 71 atomic mass units, suggesting allylation of the cysteine residues. Consistently, purified human deoxyhemoglobin reacted with chemically pure diallyl disulfide, showing the same modification as garlic extracts. Tandem mass spectrometry analysis demonstrated that garlic extract and diallyl disulfide modified hemoglobin's beta-chain at cysteine-93 (beta-93C) or cysteine-112 (beta-112C). These results indicate that garlic-derived organic disulfides as well as pure diallyl disulfide must permeate the RBC membrane and modified deoxyhemoglobin at beta-93C or beta-112C. Although the physiological role of the reported garlic extract-induced allyl modification on human hemoglobin warrants further study, the results indicate that constituents of natural products, such as those from garlic extract, modify intracellular proteins.

Full Text

Duke Authors

Cited Authors

  • Bonaventura, J; Rodriguez, EN; Beyley, V; Vega, IE

Published Date

  • August 2010

Published In

Volume / Issue

  • 13 / 4

Start / End Page

  • 943 - 949

PubMed ID

  • 20553188

Pubmed Central ID

  • PMC3132946

Electronic International Standard Serial Number (EISSN)

  • 1557-7600

International Standard Serial Number (ISSN)

  • 1096-620X

Digital Object Identifier (DOI)

  • 10.1089/jmf.2009.0258


  • eng