Identification of Limulus polyphemus haemocyanin messenger RNA.

Journal Article

Total RNA was isolated from cyanoblast-containing tissue taken from behind the compound eye of the horseshoe crab, Limulus polyphemus. Poly(A)-containing RNA separated from this by affinity chromatography on oligo(dT)-cellulose was translated in the rabbit reticulocyte haemolysate system in the presence of L-[35S]methionine. By using an antiserum to Limulus haemocyanin, polypeptides were isolated from the translation products which had a similar mobility to the authentic Limulus haemocyanin polypeptides as judged by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis.

Full Text

Duke Authors

Cited Authors

  • Wood, EJ; Bonaventura, J

Published Date

  • May 15, 1981

Published In

Volume / Issue

  • 196 / 2

Start / End Page

  • 653 - 656

PubMed ID

  • 7317005

International Standard Serial Number (ISSN)

  • 0264-6021

Language

  • eng

Conference Location

  • England