Identification of Limulus polyphemus haemocyanin messenger RNA.
Journal Article (Journal Article)
Total RNA was isolated from cyanoblast-containing tissue taken from behind the compound eye of the horseshoe crab, Limulus polyphemus. Poly(A)-containing RNA separated from this by affinity chromatography on oligo(dT)-cellulose was translated in the rabbit reticulocyte haemolysate system in the presence of L-[35S]methionine. By using an antiserum to Limulus haemocyanin, polypeptides were isolated from the translation products which had a similar mobility to the authentic Limulus haemocyanin polypeptides as judged by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis.
Full Text
Duke Authors
Cited Authors
- Wood, EJ; Bonaventura, J
Published Date
- May 1, 1981
Published In
Volume / Issue
- 196 / 2
Start / End Page
- 653 - 656
PubMed ID
- 7317005
Pubmed Central ID
- PMC1163044
Electronic International Standard Serial Number (EISSN)
- 1470-8728
International Standard Serial Number (ISSN)
- 0264-6021
Digital Object Identifier (DOI)
- 10.1042/bj1960653
Language
- eng