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The gamma2 late glycoprotein K promoter of equine herpesvirus 1 is differentially regulated by the IE and EICP0 proteins.

Publication ,  Journal Article
Kim, SK; Bowles, DE; O'callaghan, DJ
Published in: Virology
April 10, 1999

The equine herpesvirus 1 immediate-early (IE) phosphoprotein is essential for the activation of transcription from viral early and late promoters and trans-represses its own promoter. Transient-transfection assays showed that the IE protein trans-represses the gamma2 late gK promoter. Gel shift and DNase I footprinting assays demonstrated that the IE protein binds to the gK promoter sequences from -42 to -26 and from -13 to +12 that overlap the transcription initiation site (+1). These results indicated that the IE protein binds to the transcription initiation site of the gK promoter sequences, thereby repressing transcription. On the other hand, the EICP0 protein trans-activates the gamma2 late gK promoter [Bowles, D. E., Holden, V. R., Zhao, Y., and O'Callaghan, D. J. (1997). The ICP0 protein of equine herpesvirus 1 is an early protein that independently transactivates expression of all classes of viral promoters. J. Virol. 71, 4904-4914]. Overall, the EICP0 protein is able to release the gK promoter from the repressive effects of the IE protein. It has not been previously demonstrated that the major immediate-early transcriptional regulator of a herpesvirus represses expression of a late gene during infection.

Duke Scholars

Published In

Virology

DOI

ISSN

0042-6822

Publication Date

April 10, 1999

Volume

256

Issue

2

Start / End Page

173 / 179

Location

United States

Related Subject Headings

  • Virology
  • Viral Proteins
  • Transcription, Genetic
  • Trans-Activators
  • Repressor Proteins
  • Promoter Regions, Genetic
  • Immediate-Early Proteins
  • Herpesvirus 1, Equid
  • Gene Expression Regulation, Viral
  • Equidae
 

Citation

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Kim, S. K., Bowles, D. E., & O’callaghan, D. J. (1999). The gamma2 late glycoprotein K promoter of equine herpesvirus 1 is differentially regulated by the IE and EICP0 proteins. Virology, 256(2), 173–179. https://doi.org/10.1006/viro.1999.9608
Kim, S. K., D. E. Bowles, and D. J. O’callaghan. “The gamma2 late glycoprotein K promoter of equine herpesvirus 1 is differentially regulated by the IE and EICP0 proteins.Virology 256, no. 2 (April 10, 1999): 173–79. https://doi.org/10.1006/viro.1999.9608.
Kim, S. K., et al. “The gamma2 late glycoprotein K promoter of equine herpesvirus 1 is differentially regulated by the IE and EICP0 proteins.Virology, vol. 256, no. 2, Apr. 1999, pp. 173–79. Pubmed, doi:10.1006/viro.1999.9608.
Journal cover image

Published In

Virology

DOI

ISSN

0042-6822

Publication Date

April 10, 1999

Volume

256

Issue

2

Start / End Page

173 / 179

Location

United States

Related Subject Headings

  • Virology
  • Viral Proteins
  • Transcription, Genetic
  • Trans-Activators
  • Repressor Proteins
  • Promoter Regions, Genetic
  • Immediate-Early Proteins
  • Herpesvirus 1, Equid
  • Gene Expression Regulation, Viral
  • Equidae