Identification of fluorescent beads using a coded aperture snapshot spectral imager.

Published

Journal Article

We apply a coded aperture snapshot spectral imager (CASSI) to fluorescence microscopy. CASSI records a two-dimensional (2D) spectrally filtered projection of a three-dimensional (3D) spectral data cube. We minimize a convex quadratic function with total variation (TV) constraints for data cube estimation from the 2D snapshot. We adapt the TV minimization algorithm for direct fluorescent bead identification from CASSI measurements by combining a priori knowledge of the spectra associated with each bead type. Our proposed method creates a 2D bead identity image. Simulated fluorescence CASSI measurements are used to evaluate the behavior of the algorithm. We also record real CASSI measurements of a ten bead type fluorescence scene and create a 2D bead identity map. A baseline image from filtered-array imaging system verifies CASSI's 2D bead identity map.

Full Text

Duke Authors

Cited Authors

  • Cull, CF; Choi, K; Brady, DJ; Oliver, T

Published Date

  • April 2010

Published In

Volume / Issue

  • 49 / 10

Start / End Page

  • B59 - B70

PubMed ID

  • 20357842

Pubmed Central ID

  • 20357842

Electronic International Standard Serial Number (EISSN)

  • 1539-4522

International Standard Serial Number (ISSN)

  • 0003-6935

Digital Object Identifier (DOI)

  • 10.1364/ao.49.000b59

Language

  • eng