A structure-based mechanism for drug binding by multidrug transporters.

Journal Article (Journal Article;Review)

Multidrug transporters bind chemically dissimilar, potentially cytotoxic compounds and remove them from the cell. How these transporters carry out either of these functions is unknown. On the basis of crystal structures of the multidrug-binding domain of the transcription activator BmrR and mutagenesis studies on the bacterial multidrug transporter MdfA, we propose a possible mechanism for the binding of cationic lipophilic drugs by multidrug transporters. The key element of this mechanism includes a conformational change in the transporter that exposes a buried charged residue in the substrate-binding pocket and allows access to this site by only those drugs that are its steric and electrostatic complements.

Full Text

Duke Authors

Cited Authors

  • Zheleznova, EE; Markham, P; Edgar, R; Bibi, E; Neyfakh, AA; Brennan, RG

Published Date

  • February 2000

Published In

Volume / Issue

  • 25 / 2

Start / End Page

  • 39 - 43

PubMed ID

  • 10664577

International Standard Serial Number (ISSN)

  • 0968-0004

Digital Object Identifier (DOI)

  • 10.1016/s0968-0004(99)01514-5


  • eng

Conference Location

  • England