Characterization of the DNA- and metal-binding properties of Vibrio anguillarum fur reveals conservation of a structural Zn(2+) ion.
Publication
, Journal Article
Zheleznova, EE; Crosa, JH; Brennan, RG
Published in: J Bacteriol
November 2000
The ferric uptake regulator, Fur, represses iron uptake and siderophore biosynthetic genes under iron-replete conditions. Here we report in vitro solution studies on Vibrio anguillarum Fur binding to the consensus 19-bp Escherichia coli iron box in the presence of several divalent metals. We found that V. anguillarum Fur binds the iron box in the presence of Mn(2+), Co(2+), Cd(2+), and to a lesser extent Ni(2+) but, unlike E. coli Fur, not in the presence of Zn(2+). We also found that V. anguillarum Fur contains a structural zinc ion that is necessary yet alone is insufficient for DNA binding.
Duke Scholars
Published In
J Bacteriol
DOI
ISSN
0021-9193
Publication Date
November 2000
Volume
182
Issue
21
Start / End Page
6264 / 6267
Location
United States
Related Subject Headings
- Zinc
- Vibrio
- Sequence Alignment
- Repressor Proteins
- Protein Binding
- Nickel
- Molecular Sequence Data
- Microbiology
- Manganese
- Genes, Bacterial
Citation
APA
Chicago
ICMJE
MLA
NLM
Zheleznova, E. E., Crosa, J. H., & Brennan, R. G. (2000). Characterization of the DNA- and metal-binding properties of Vibrio anguillarum fur reveals conservation of a structural Zn(2+) ion. J Bacteriol, 182(21), 6264–6267. https://doi.org/10.1128/JB.182.21.6264-6267.2000
Zheleznova, E. E., J. H. Crosa, and R. G. Brennan. “Characterization of the DNA- and metal-binding properties of Vibrio anguillarum fur reveals conservation of a structural Zn(2+) ion.” J Bacteriol 182, no. 21 (November 2000): 6264–67. https://doi.org/10.1128/JB.182.21.6264-6267.2000.
Zheleznova EE, Crosa JH, Brennan RG. Characterization of the DNA- and metal-binding properties of Vibrio anguillarum fur reveals conservation of a structural Zn(2+) ion. J Bacteriol. 2000 Nov;182(21):6264–7.
Zheleznova, E. E., et al. “Characterization of the DNA- and metal-binding properties of Vibrio anguillarum fur reveals conservation of a structural Zn(2+) ion.” J Bacteriol, vol. 182, no. 21, Nov. 2000, pp. 6264–67. Pubmed, doi:10.1128/JB.182.21.6264-6267.2000.
Zheleznova EE, Crosa JH, Brennan RG. Characterization of the DNA- and metal-binding properties of Vibrio anguillarum fur reveals conservation of a structural Zn(2+) ion. J Bacteriol. 2000 Nov;182(21):6264–6267.
Published In
J Bacteriol
DOI
ISSN
0021-9193
Publication Date
November 2000
Volume
182
Issue
21
Start / End Page
6264 / 6267
Location
United States
Related Subject Headings
- Zinc
- Vibrio
- Sequence Alignment
- Repressor Proteins
- Protein Binding
- Nickel
- Molecular Sequence Data
- Microbiology
- Manganese
- Genes, Bacterial