Characterization of the DNA- and metal-binding properties of Vibrio anguillarum fur reveals conservation of a structural Zn(2+) ion.

Journal Article (Journal Article)

The ferric uptake regulator, Fur, represses iron uptake and siderophore biosynthetic genes under iron-replete conditions. Here we report in vitro solution studies on Vibrio anguillarum Fur binding to the consensus 19-bp Escherichia coli iron box in the presence of several divalent metals. We found that V. anguillarum Fur binds the iron box in the presence of Mn(2+), Co(2+), Cd(2+), and to a lesser extent Ni(2+) but, unlike E. coli Fur, not in the presence of Zn(2+). We also found that V. anguillarum Fur contains a structural zinc ion that is necessary yet alone is insufficient for DNA binding.

Full Text

Duke Authors

Cited Authors

  • Zheleznova, EE; Crosa, JH; Brennan, RG

Published Date

  • November 2000

Published In

Volume / Issue

  • 182 / 21

Start / End Page

  • 6264 - 6267

PubMed ID

  • 11029454

Pubmed Central ID

  • PMC94768

International Standard Serial Number (ISSN)

  • 0021-9193

Digital Object Identifier (DOI)

  • 10.1128/JB.182.21.6264-6267.2000

Language

  • eng

Conference Location

  • United States