Characterization of the DNA- and metal-binding properties of Vibrio anguillarum fur reveals conservation of a structural Zn(2+) ion.
Journal Article (Journal Article)
The ferric uptake regulator, Fur, represses iron uptake and siderophore biosynthetic genes under iron-replete conditions. Here we report in vitro solution studies on Vibrio anguillarum Fur binding to the consensus 19-bp Escherichia coli iron box in the presence of several divalent metals. We found that V. anguillarum Fur binds the iron box in the presence of Mn(2+), Co(2+), Cd(2+), and to a lesser extent Ni(2+) but, unlike E. coli Fur, not in the presence of Zn(2+). We also found that V. anguillarum Fur contains a structural zinc ion that is necessary yet alone is insufficient for DNA binding.
Full Text
Duke Authors
Cited Authors
- Zheleznova, EE; Crosa, JH; Brennan, RG
Published Date
- November 2000
Published In
Volume / Issue
- 182 / 21
Start / End Page
- 6264 - 6267
PubMed ID
- 11029454
Pubmed Central ID
- PMC94768
International Standard Serial Number (ISSN)
- 0021-9193
Digital Object Identifier (DOI)
- 10.1128/JB.182.21.6264-6267.2000
Language
- eng
Conference Location
- United States