Crystal structure of the lactose operon repressor and its complexes with DNA and inducer.

Published

Journal Article

The lac operon of Escherichia coli is the paradigm for gene regulation. Its key component is the lac repressor, a product of the lacI gene. The three-dimensional structures of the intact lac repressor, the lac repressor bound to the gratuitous inducer isopropyl-beta-D-1-thiogalactoside (IPTG) and the lac repressor complexed with a 21-base pair symmetric operator DNA have been determined. These three structures show the conformation of the molecule in both the induced and repressed states and provide a framework for understanding a wealth of biochemical and genetic information. The DNA sequence of the lac operon has three lac repressor recognition sites in a stretch of 500 base pairs. The crystallographic structure of the complex with DNA suggests that the tetrameric repressor functions synergistically with catabolite gene activator protein (CAP) and participates in the quaternary formation of repression loops in which one tetrameric repressor interacts simultaneously with two sites on the genomic DNA.

Full Text

Duke Authors

Cited Authors

  • Lewis, M; Chang, G; Horton, NC; Kercher, MA; Pace, HC; Schumacher, MA; Brennan, RG; Lu, P

Published Date

  • March 1996

Published In

Volume / Issue

  • 271 / 5253

Start / End Page

  • 1247 - 1254

PubMed ID

  • 8638105

Pubmed Central ID

  • 8638105

Electronic International Standard Serial Number (EISSN)

  • 1095-9203

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.271.5253.1247

Language

  • eng