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Nuclear protein CBP is a coactivator for the transcription factor CREB.

Publication ,  Journal Article
Kwok, RP; Lundblad, JR; Chrivia, JC; Richards, JP; Bächinger, HP; Brennan, RG; Roberts, SG; Green, MR; Goodman, RH
Published in: Nature
July 21, 1994

The transcription factor CREB binds to a DNA element known as the cAMP-regulated enhancer (CRE). CREB is activated through phosphorylation by protein kinase A (PKA), but precisely how phosphorylation stimulates CREB function is unknown. One model is that phosphorylation may allow the recruitment of coactivators which then interact with basal transcription factors. We have previously identified a nuclear protein of M(r)265K, CBP, that binds specifically to the PKA-phosphorylated form of CREB. We have used fluorescence anisotropy measurements to define the equilibrium binding parameters of the phosphoCREB:CBP interaction and report here that CBP can activate transcription through a region in its carboxy terminus. The activation domain of CBP interacts with the basal transcription factor TFIIB through a domain that is conserved in the yeast coactivator ADA-1 (ref. 8). Consistent with its role as a coactivator, CBP augments the activity of phosphorylated CREB to activate transcription of cAMP-responsive genes.

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Published In

Nature

DOI

ISSN

0028-0836

Publication Date

July 21, 1994

Volume

370

Issue

6486

Start / End Page

223 / 226

Location

England

Related Subject Headings

  • Zinc Fingers
  • Tumor Cells, Cultured
  • Transcription, Genetic
  • Transcription Factors
  • Transcription Factor TFIIB
  • Trans-Activators
  • Somatostatin
  • Recombinant Fusion Proteins
  • Protein Binding
  • Phosphorylation
 

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Kwok, R. P., Lundblad, J. R., Chrivia, J. C., Richards, J. P., Bächinger, H. P., Brennan, R. G., … Goodman, R. H. (1994). Nuclear protein CBP is a coactivator for the transcription factor CREB. Nature, 370(6486), 223–226. https://doi.org/10.1038/370223a0
Kwok, R. P., J. R. Lundblad, J. C. Chrivia, J. P. Richards, H. P. Bächinger, R. G. Brennan, S. G. Roberts, M. R. Green, and R. H. Goodman. “Nuclear protein CBP is a coactivator for the transcription factor CREB.Nature 370, no. 6486 (July 21, 1994): 223–26. https://doi.org/10.1038/370223a0.
Kwok RP, Lundblad JR, Chrivia JC, Richards JP, Bächinger HP, Brennan RG, et al. Nuclear protein CBP is a coactivator for the transcription factor CREB. Nature. 1994 Jul 21;370(6486):223–6.
Kwok, R. P., et al. “Nuclear protein CBP is a coactivator for the transcription factor CREB.Nature, vol. 370, no. 6486, July 1994, pp. 223–26. Pubmed, doi:10.1038/370223a0.
Kwok RP, Lundblad JR, Chrivia JC, Richards JP, Bächinger HP, Brennan RG, Roberts SG, Green MR, Goodman RH. Nuclear protein CBP is a coactivator for the transcription factor CREB. Nature. 1994 Jul 21;370(6486):223–226.
Journal cover image

Published In

Nature

DOI

ISSN

0028-0836

Publication Date

July 21, 1994

Volume

370

Issue

6486

Start / End Page

223 / 226

Location

England

Related Subject Headings

  • Zinc Fingers
  • Tumor Cells, Cultured
  • Transcription, Genetic
  • Transcription Factors
  • Transcription Factor TFIIB
  • Trans-Activators
  • Somatostatin
  • Recombinant Fusion Proteins
  • Protein Binding
  • Phosphorylation