Targeted lymphoid homing of dendritic cells is required for prolongation of allograft survival.

Published

Journal Article

Accumulating evidence that dendritic cells (DC) are important regulators of peripheral immune tolerance has led to the concept that donor-derived DC may be useful for inducing donor-specific transplantation tolerance. Although in vitro studies in this field have been encouraging, in vivo results have been inconsistent. Recent evidence has suggested a critical role of lymphoid organs in tolerance induction. In this study, we use a novel gene transduction technique to show that engineered expression of CCR7 on immature DC can markedly increase DC homing to lymphoid organs, leading to increased interaction with Ag-specific T cells. Moreover, we show that a single infusion of DC coexpressing CCR7 and the immunomodulatory molecule viral IL-10 (vIL-10) markedly prolongs cardiac allograft survival (mean survival time >100 days); importantly, DC expressing either vIL-10 alone or CCR7 alone was not effective. These results demonstrate an important paradigm for immune modulation using DC.

Full Text

Cited Authors

  • Garrod, KR; Chang, CK; Liu, F-C; Brennan, TV; Foster, RD; Kang, S-M

Published Date

  • July 2006

Published In

Volume / Issue

  • 177 / 2

Start / End Page

  • 863 - 868

PubMed ID

  • 16818740

Pubmed Central ID

  • 16818740

Electronic International Standard Serial Number (EISSN)

  • 1550-6606

International Standard Serial Number (ISSN)

  • 0022-1767

Digital Object Identifier (DOI)

  • 10.4049/jimmunol.177.2.863

Language

  • eng