Membrane compartmentation is required for efficient T cell activation.


Journal Article

The plasma membrane of mammalian cells contains detergent-resistant membrane rafts enriched in glycosphingolipids and cholesterol. Although several important signaling molecules have been found in such rafts, evidence documenting a functional role for their localization has been scarce. Using a fractionation scheme that preserves tyrosine phosphorylation, we show that T cell activation leads to a striking compartmentation in the rafts of activated T cell receptor and associated signal-transducing molecules. Conditions that reversibly disrupt raft structure either by dispersing their contents or by forcing their internalization reversibly disrupt the earliest steps of T cell activation. Thus, raft integrity is a prerequisite for efficient T cell receptor signal transduction.

Full Text

Cited Authors

  • Xavier, R; Brennan, T; Li, Q; McCormack, C; Seed, B

Published Date

  • June 1998

Published In

Volume / Issue

  • 8 / 6

Start / End Page

  • 723 - 732

PubMed ID

  • 9655486

Pubmed Central ID

  • 9655486

Electronic International Standard Serial Number (EISSN)

  • 1097-4180

International Standard Serial Number (ISSN)

  • 1074-7613

Digital Object Identifier (DOI)

  • 10.1016/s1074-7613(00)80577-4


  • eng