Alteration of Caenorhabditis elegans gene expression by targeted transformation.

Published

Journal Article

We have produced strains carrying a synthetic fusion of parts of two vitellogenin genes, vit-2 and vit-6, integrated into the Caenorhabditis elegans genome. In most of the 63 transformant strains, the plasmid sequences are integrated at random locations in the genome. However, in two strains the transgene integrated by homologous recombination into the endogenous vit-2 gene. In both cases the reciprocal exchange between the chromosome and the injected circular plasmid containing a promoter deletion led to switching of the plasmid-borne promoter and the endogenous promoter, with a reduction in vit-2 expression. Thus in nematodes, transforming DNA can integrate by homologous recombination to result in partial inactivation of the chromosomal locus. The simplicity of the event and its reasonably high frequency suggest that gene targeting by homologous recombination should be considered as a method for directed inactivation of C. elegans genes.

Full Text

Duke Authors

Cited Authors

  • Broverman, S; MacMorris, M; Blumenthal, T

Published Date

  • May 1993

Published In

Volume / Issue

  • 90 / 10

Start / End Page

  • 4359 - 4363

PubMed ID

  • 8506273

Pubmed Central ID

  • 8506273

Electronic International Standard Serial Number (EISSN)

  • 1091-6490

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.90.10.4359

Language

  • eng