Skip to main content
Journal cover image

Proinflammatory cytokine expression profile in degenerated and herniated human intervertebral disc tissues.

Publication ,  Journal Article
Shamji, MF; Setton, LA; Jarvis, W; So, S; Chen, J; Jing, L; Bullock, R; Isaacs, RE; Brown, C; Richardson, WJ
Published in: Arthritis Rheum
July 2010

OBJECTIVE: Prior reports document macrophage and lymphocyte infiltration with proinflammatory cytokine expression in pathologic intervertebral disc (IVD) tissues. Nevertheless, the role of the Th17 lymphocyte lineage in mediating disc disease remains uninvestigated. We undertook this study to evaluate the immunophenotype of pathologic IVD specimens, including interleukin-17 (IL-17) expression, from surgically obtained IVD tissue and from nondegenerated autopsy control tissue. METHODS: Surgical IVD tissues were procured from patients with degenerative disc disease (n = 25) or herniated IVDs (n = 12); nondegenerated autopsy control tissue was also obtained (n = 8) from the anulus fibrosus and nucleus pulposus regions. Immunohistochemistry was performed for cell surface antigens (CD68 for macrophages, CD4 for lymphocytes) and various cytokines, with differences in cellularity and target immunoreactivity scores analyzed between surgical tissue groups and between autopsy control tissue regions. RESULTS: Immunoreactivity for IL-4, IL-6, IL-12, and interferon-gamma (IFNgamma) was modest in surgical IVD tissue, although expression was higher in herniated IVD samples and virtually nonexistent in control samples. The Th17 lymphocyte product IL-17 was present in >70% of surgical tissue fields, and among control samples was detected rarely in anulus fibrosus regions and modestly in nucleus pulposus regions. Macrophages were prevalent in surgical tissues, particularly herniated IVD samples, and lymphocytes were expectedly scarce. Control tissue revealed lesser infiltration by macrophages and a near absence of lymphocytes. CONCLUSION: Greater IFNgamma positivity, macrophage presence, and cellularity in herniated IVDs suggests a pattern of Th1 lymphocyte activation in this pathology. Remarkable pathologic IVD tissue expression of IL-17 is a novel finding that contrasts markedly with low levels of IL-17 in autopsy control tissue. These findings suggest involvement of Th17 lymphocytes in the pathomechanism of disc degeneration.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Arthritis Rheum

DOI

EISSN

1529-0131

Publication Date

July 2010

Volume

62

Issue

7

Start / End Page

1974 / 1982

Location

United States

Related Subject Headings

  • T-Lymphocytes, Helper-Inducer
  • Middle Aged
  • Male
  • Macrophages
  • Lymphocyte Subsets
  • Lymphocyte Activation
  • Intervertebral Disc Displacement
  • Intervertebral Disc Degeneration
  • Interleukin-17
  • Humans
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Shamji, M. F., Setton, L. A., Jarvis, W., So, S., Chen, J., Jing, L., … Richardson, W. J. (2010). Proinflammatory cytokine expression profile in degenerated and herniated human intervertebral disc tissues. Arthritis Rheum, 62(7), 1974–1982. https://doi.org/10.1002/art.27444
Shamji, Mohammed F., Lori A. Setton, Wingrove Jarvis, Stephen So, Jun Chen, Liufang Jing, Robert Bullock, Robert E. Isaacs, Christopher Brown, and William J. Richardson. “Proinflammatory cytokine expression profile in degenerated and herniated human intervertebral disc tissues.Arthritis Rheum 62, no. 7 (July 2010): 1974–82. https://doi.org/10.1002/art.27444.
Shamji MF, Setton LA, Jarvis W, So S, Chen J, Jing L, et al. Proinflammatory cytokine expression profile in degenerated and herniated human intervertebral disc tissues. Arthritis Rheum. 2010 Jul;62(7):1974–82.
Shamji, Mohammed F., et al. “Proinflammatory cytokine expression profile in degenerated and herniated human intervertebral disc tissues.Arthritis Rheum, vol. 62, no. 7, July 2010, pp. 1974–82. Pubmed, doi:10.1002/art.27444.
Shamji MF, Setton LA, Jarvis W, So S, Chen J, Jing L, Bullock R, Isaacs RE, Brown C, Richardson WJ. Proinflammatory cytokine expression profile in degenerated and herniated human intervertebral disc tissues. Arthritis Rheum. 2010 Jul;62(7):1974–1982.
Journal cover image

Published In

Arthritis Rheum

DOI

EISSN

1529-0131

Publication Date

July 2010

Volume

62

Issue

7

Start / End Page

1974 / 1982

Location

United States

Related Subject Headings

  • T-Lymphocytes, Helper-Inducer
  • Middle Aged
  • Male
  • Macrophages
  • Lymphocyte Subsets
  • Lymphocyte Activation
  • Intervertebral Disc Displacement
  • Intervertebral Disc Degeneration
  • Interleukin-17
  • Humans