1,25-Dihydroxyvitamin D3 stimulates avian and mammalian cartilage growth in vitro.

Journal Article

We addressed the question of whether 1,25-dihydroxyvitamin D3 (1,25-(OH)2D) could directly stimulate cartilage growth in vitro. Pelvic leaflets from chick embryos and scapular growth plates from fetal pigs were organ cultured in serum-free medium in the presence and absence of 1,25-(OH)2D. After 3 days of incubation, 1,25-(OH)2D had increased the pelvic cartilage wet weight 42% and the dry weight 32% above the weight of cartilages incubated in medium alone. 1,25-(OH)2D (10(-9) M-10(-12) M) caused a dose-dependent increase in weight, with maximal increases at 10(-9) M. Furthermore, two deuterized derivatives of 1,25-(OH)2D, 26,27-D6-1,25-(OH)2D3 and 24,26,27-D8-1,25-(OH)2D3, stimulated pelvic cartilage growth in vitro. 26,27-D6-1,25-(OH)2D stimulated increases in growth plate weight above growth plates incubated in medium alone. 26,27-D6-1,25-(OH)2D3 appeared to be potent at lower concentrations than 1,25-(OH)2D on growth plate cartilage. Thus, 1,25-(OH)2D stimulated in vitro growth in two growing cartilage models, the avian pelvic cartilage and the mammalian scapular growth plate cartilage.

Full Text

Duke Authors

Cited Authors

  • Burch, WM; Lopez-Claros, M; Uskokovic, MR; Drezner, MK

Published Date

  • February 1, 1988

Published In

Volume / Issue

  • 3 / 1

Start / End Page

  • 87 - 91

PubMed ID

  • 3213605

International Standard Serial Number (ISSN)

  • 0884-0431

Digital Object Identifier (DOI)

  • 10.1002/jbmr.5650030113

Language

  • eng

Conference Location

  • United States