Whole-cell-analysis of live cardiomyocytes using wide-field interferometric phase microscopy.


Journal Article

We apply wide-field interferometric microscopy techniques to acquire quantitative phase profiles of ventricular cardiomyocytes in vitro during their rapid contraction with high temporal and spatial resolution. The whole-cell phase profiles are analyzed to yield valuable quantitative parameters characterizing the cell dynamics, without the need to decouple thickness from refractive index differences. Our experimental results verify that these new parameters can be used with wide field interferometric microscopy to discriminate the modulation of cardiomyocyte contraction dynamics due to temperature variation. To demonstrate the necessity of the proposed numerical analysis for cardiomyocytes, we present confocal dual-fluorescence-channel microscopy results which show that the rapid motion of the cell organelles during contraction preclude assuming a homogenous refractive index over the entire cell contents, or using multiple-exposure or scanning microscopy.

Full Text

Duke Authors

Cited Authors

  • Shaked, NT; Satterwhite, LL; Bursac, N; Wax, A

Published Date

  • August 23, 2010

Published In

Volume / Issue

  • 1 / 2

Start / End Page

  • 706 - 719

PubMed ID

  • 21258502

Pubmed Central ID

  • 21258502

Electronic International Standard Serial Number (EISSN)

  • 2156-7085

International Standard Serial Number (ISSN)

  • 2156-7085

Digital Object Identifier (DOI)

  • 10.1364/boe.1.000706


  • eng