Reflective interferometric chamber for quantitative phase imaging of biological sample dynamics.

Journal Article (Letter)

We introduce a new interferometric setup for single-exposure wide-field holographic phase imaging of highly dynamic biological samples. In this setup, the interferometric signal originates from a specially designed reflective interferometric chamber (InCh), creating an off-axis interferogram on the output plane of the system. The setup only requires the InCh and a simple reflection-mode two lens imaging system, without the need for additional optical elements such as gratings in the beam path. In addition, due to the close-to-common-path geometry of the setup, phase noise is greatly reduced. We experimentally compare the inherent phase stability of the system in ambient conditions to that of a conventional interferometer. We also demonstrate use of this system for wide-field quantitative phase imaging of two different highly dynamic, optically transparent biological samples: beating myocardial cells and moving unicellular microorganisms.

Full Text

Duke Authors

Cited Authors

  • Shaked, NT; Zhu, Y; Badie, N; Bursac, N; Wax, A

Published Date

  • May 2010

Published In

Volume / Issue

  • 15 / 3

Start / End Page

  • 030503 -

PubMed ID

  • 20614989

Pubmed Central ID

  • PMC4588377

Electronic International Standard Serial Number (EISSN)

  • 1560-2281

International Standard Serial Number (ISSN)

  • 1083-3668

Digital Object Identifier (DOI)

  • 10.1117/1.3420179


  • eng