Skip to main content

Implantation of mouse embryonic stem cell-derived cardiac progenitor cells preserves function of infarcted murine hearts.

Publication ,  Journal Article
Christoforou, N; Oskouei, BN; Esteso, P; Hill, CM; Zimmet, JM; Bian, W; Bursac, N; Leong, KW; Hare, JM; Gearhart, JD
Published in: PloS one
July 2010

Stem cell transplantation holds great promise for the treatment of myocardial infarction injury. We recently described the embryonic stem cell-derived cardiac progenitor cells (CPCs) capable of differentiating into cardiomyocytes, vascular endothelium, and smooth muscle. In this study, we hypothesized that transplanted CPCs will preserve function of the infarcted heart by participating in both muscle replacement and neovascularization. Differentiated CPCs formed functional electromechanical junctions with cardiomyocytes in vitro and conducted action potentials over cm-scale distances. When transplanted into infarcted mouse hearts, CPCs engrafted long-term in the infarct zone and surrounding myocardium without causing teratomas or arrhythmias. The grafted cells differentiated into cross-striated cardiomyocytes forming gap junctions with the host cells, while also contributing to neovascularization. Serial echocardiography and pressure-volume catheterization demonstrated attenuated ventricular dilatation and preserved left ventricular fractional shortening, systolic and diastolic function. Our results demonstrate that CPCs can engraft, differentiate, and preserve the functional output of the infarcted heart.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

PloS one

DOI

EISSN

1932-6203

ISSN

1932-6203

Publication Date

July 2010

Volume

5

Issue

7

Start / End Page

e11536

Related Subject Headings

  • Stem Cell Transplantation
  • Reverse Transcriptase Polymerase Chain Reaction
  • Myocytes, Cardiac
  • Myocardial Infarction
  • Mice
  • Kaplan-Meier Estimate
  • General Science & Technology
  • Female
  • Embryonic Stem Cells
  • Echocardiography
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Christoforou, N., Oskouei, B. N., Esteso, P., Hill, C. M., Zimmet, J. M., Bian, W., … Gearhart, J. D. (2010). Implantation of mouse embryonic stem cell-derived cardiac progenitor cells preserves function of infarcted murine hearts. PloS One, 5(7), e11536. https://doi.org/10.1371/journal.pone.0011536
Christoforou, Nicolas, Behzad N. Oskouei, Paul Esteso, Christine M. Hill, Jeffrey M. Zimmet, Weining Bian, Nenad Bursac, Kam W. Leong, Joshua M. Hare, and John D. Gearhart. “Implantation of mouse embryonic stem cell-derived cardiac progenitor cells preserves function of infarcted murine hearts.PloS One 5, no. 7 (July 2010): e11536. https://doi.org/10.1371/journal.pone.0011536.
Christoforou N, Oskouei BN, Esteso P, Hill CM, Zimmet JM, Bian W, et al. Implantation of mouse embryonic stem cell-derived cardiac progenitor cells preserves function of infarcted murine hearts. PloS one. 2010 Jul;5(7):e11536.
Christoforou, Nicolas, et al. “Implantation of mouse embryonic stem cell-derived cardiac progenitor cells preserves function of infarcted murine hearts.PloS One, vol. 5, no. 7, July 2010, p. e11536. Epmc, doi:10.1371/journal.pone.0011536.
Christoforou N, Oskouei BN, Esteso P, Hill CM, Zimmet JM, Bian W, Bursac N, Leong KW, Hare JM, Gearhart JD. Implantation of mouse embryonic stem cell-derived cardiac progenitor cells preserves function of infarcted murine hearts. PloS one. 2010 Jul;5(7):e11536.

Published In

PloS one

DOI

EISSN

1932-6203

ISSN

1932-6203

Publication Date

July 2010

Volume

5

Issue

7

Start / End Page

e11536

Related Subject Headings

  • Stem Cell Transplantation
  • Reverse Transcriptase Polymerase Chain Reaction
  • Myocytes, Cardiac
  • Myocardial Infarction
  • Mice
  • Kaplan-Meier Estimate
  • General Science & Technology
  • Female
  • Embryonic Stem Cells
  • Echocardiography