Mesoscopic hydrogel molding to control the 3D geometry of bioartificial muscle tissues.

Journal Article (Journal Article)

This protocol describes a cell/hydrogel molding method for precise and reproducible biomimetic fabrication of three-dimensional (3D) muscle tissue architectures in vitro. Using a high aspect ratio soft lithography technique, we fabricate polydimethylsiloxane (PDMS) molds containing arrays of mesoscopic posts with defined size, elongation and spacing. On cell/hydrogel molding, these posts serve to enhance the diffusion of nutrients to cells by introducing elliptical pores in the cell-laden hydrogels and to guide local 3D cell alignment by governing the spatial pattern of mechanical tension. Instead of ultraviolet or chemical cross-linking, this method utilizes natural hydrogel polymerization and topographically constrained cell-mediated gel compaction to create the desired 3D tissue structures. We apply this method to fabricate several square centimeter large, few hundred micron-thick bioartificial muscle tissues composed of viable, dense, uniformly aligned and highly differentiated cardiac or skeletal muscle fibers. The protocol takes 4-5 d to fabricate PDMS molds followed by 2 weeks of cell culture.

Full Text

Duke Authors

Cited Authors

  • Bian, W; Liau, B; Badie, N; Bursac, N

Published Date

  • January 2009

Published In

Volume / Issue

  • 4 / 10

Start / End Page

  • 1522 - 1534

PubMed ID

  • 19798085

Pubmed Central ID

  • PMC2924624

Electronic International Standard Serial Number (EISSN)

  • 1750-2799

International Standard Serial Number (ISSN)

  • 1754-2189

Digital Object Identifier (DOI)

  • 10.1038/nprot.2009.155


  • eng