We present a pilot-assisted method for estimating the frequency selective channel in a MIMO-OFDM (Multiple Input Multiple Output - Orthogonal Frequency Division Multiplexing) system. The pilot sequence is designed using the DFT (Discrete Fourier Transform) of the Golay complementary sequences. Novel exploitation of the perfect autocorrelation property of the Golay codes, in conjunction with OSTBC (Orthogonal Space-Time Block Code) based pilot waveform scheduling across multiple OFDM frames, facilitates simple separation of the channel mixtures at the receive antennas. The DFT length used to transform the complementary sequence into the frequency domain is shown to be a key critical parameter for correctly estimating the channel. NMSE (Normalized Mean Squared Error) between the actual and the estimated channel is used to characterize the estimation performance. © 2008 IEEE.