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Four-dimensional analysis of vascularization during primary development of an organ, the gonad.

Publication ,  Journal Article
Coveney, D; Cool, J; Oliver, T; Capel, B
Published in: Proc Natl Acad Sci U S A
May 20, 2008

Time-lapse microscopy has advanced our understanding of yolk sac and early embryonic vascularization. However, it has been difficult to assess endothelial interactions during epithelial morphogenesis of internal organs. To address this issue we have developed the first time-lapse system to study vascularization of a mammalian organ in four dimensions. We show that vascularization of XX and XY gonads is a highly dynamic, sexually dimorphic process. The XX gonad recruits vasculature by a typical angiogenic process. In contrast, the XY gonad recruits and patterns vasculature by a novel remodeling mechanism beginning with breakdown of an existing mesonephric vessel. Subsequently, in XY organs individual endothelial cells migrate and reaggregate in the coelomic domain to form the major testicular artery. Migrating endothelial cells respect domain boundaries well before they are morphologically evident, subdividing the gonad into 10 avascular regions where testis cords form. This model of vascular development in an internal organ has a direct impact on the current dogma of vascular integration during organ development and presents important parallels with mechanisms of tumor vascularization.

Duke Scholars

Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

May 20, 2008

Volume

105

Issue

20

Start / End Page

7212 / 7217

Location

United States

Related Subject Headings

  • Time Factors
  • Testis
  • Ovary
  • Organ Culture Techniques
  • Neovascularization, Physiologic
  • Mice, Transgenic
  • Mice
  • Male
  • Gonads
  • Germ Cells
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Coveney, D., Cool, J., Oliver, T., & Capel, B. (2008). Four-dimensional analysis of vascularization during primary development of an organ, the gonad. Proc Natl Acad Sci U S A, 105(20), 7212–7217. https://doi.org/10.1073/pnas.0707674105
Coveney, Douglas, Jonah Cool, Tim Oliver, and Blanche Capel. “Four-dimensional analysis of vascularization during primary development of an organ, the gonad.Proc Natl Acad Sci U S A 105, no. 20 (May 20, 2008): 7212–17. https://doi.org/10.1073/pnas.0707674105.
Coveney D, Cool J, Oliver T, Capel B. Four-dimensional analysis of vascularization during primary development of an organ, the gonad. Proc Natl Acad Sci U S A. 2008 May 20;105(20):7212–7.
Coveney, Douglas, et al. “Four-dimensional analysis of vascularization during primary development of an organ, the gonad.Proc Natl Acad Sci U S A, vol. 105, no. 20, May 2008, pp. 7212–17. Pubmed, doi:10.1073/pnas.0707674105.
Coveney D, Cool J, Oliver T, Capel B. Four-dimensional analysis of vascularization during primary development of an organ, the gonad. Proc Natl Acad Sci U S A. 2008 May 20;105(20):7212–7217.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

May 20, 2008

Volume

105

Issue

20

Start / End Page

7212 / 7217

Location

United States

Related Subject Headings

  • Time Factors
  • Testis
  • Ovary
  • Organ Culture Techniques
  • Neovascularization, Physiologic
  • Mice, Transgenic
  • Mice
  • Male
  • Gonads
  • Germ Cells