Reduced arsenic clearance and increased toxicity in aquaglyceroporin-9-null mice.

Published

Journal Article

Expressed in liver, aquaglyceroporin-9 (AQP9) is permeated by glycerol, arsenite, and other small, neutral solutes. To evaluate a possible protective role, AQP9-null mice were evaluated for in vivo arsenic toxicity. After injection with NaAsO(2), AQP9-null mice suffer reduced survival rates (LD(50), 12 mg/kg) compared with WT mice (LD(50), 15 mg/kg). The highest tissue level of arsenic is in heart, with AQP9-null mice accumulating 10-20 times more arsenic than WT mice. Within hours after NaAsO(2) injection, AQP9-null mice sustain profound bradycardia, despite normal serum electrolytes. Increased arsenic levels are also present in liver, lung, spleen, and testis of AQP9-null mice. Arsenic levels in the feces and urine of AQP9-null mice are only approximately 10% of the WT levels, and reduced clearance of multiple arsenic species by the AQP9-null mice suggests that AQP9 is involved in the export of multiple forms of arsenic. Immunohistochemical staining of liver sections revealed that AQP9 is most abundant in basolateral membrane of hepatocytes adjacent to the sinusoids. AQP9 is not detected in heart or kidney by PCR or immunohistochemistry. We propose that AQP9 provides a route for excretion of arsenic by the liver, thereby providing partial protection of the whole animal from arsenic toxicity.

Full Text

Duke Authors

Cited Authors

  • Carbrey, JM; Song, L; Zhou, Y; Yoshinaga, M; Rojek, A; Wang, Y; Liu, Y; Lujan, HL; DiCarlo, SE; Nielsen, S; Rosen, BP; Agre, P; Mukhopadhyay, R

Published Date

  • September 15, 2009

Published In

Volume / Issue

  • 106 / 37

Start / End Page

  • 15956 - 15960

PubMed ID

  • 19805235

Pubmed Central ID

  • 19805235

Electronic International Standard Serial Number (EISSN)

  • 1091-6490

Digital Object Identifier (DOI)

  • 10.1073/pnas.0908108106

Language

  • eng

Conference Location

  • United States