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Casein kinase II phosphorylates the eukaryote-specific C-terminal domain of topoisomerase II in vivo.

Publication ,  Journal Article
Cardenas, ME; Dang, Q; Glover, CV; Gasser, SM
Published in: EMBO J
May 1992

The decatenation activity of DNA topoisomerase II is essential for viability as eukaryotic cells traverse mitosis. Phosphorylation has been shown to stimulate topoisomerase II activity in vitro. Here we show that topoisomerase II is a phosphoprotein in yeast and that the level of incorporated phosphate is significantly higher at mitosis than in G1. Comparison of tryptic phosphopeptide maps reveals that the major phosphorylation sites in vivo are targets for casein kinase II. Incorporation of phosphate into topoisomerase II is nearly undetectable at the non-permissive temperature in a conditional casein kinase II mutant. The sites modified by casein kinase II are located in the extreme C-terminal domain of topoisomerase II. This domain is absent in prokaryotic and highly divergent among eukaryotic type II topoisomerases, and may serve to regulate functions of topoisomerase II that are unique to eukaryotic cells.

Duke Scholars

Published In

EMBO J

DOI

ISSN

0261-4189

Publication Date

May 1992

Volume

11

Issue

5

Start / End Page

1785 / 1796

Location

England

Related Subject Headings

  • Saccharomyces cerevisiae
  • Protein Serine-Threonine Kinases
  • Precipitin Tests
  • Phosphorylation
  • Peptide Mapping
  • Fluorescent Antibody Technique
  • Electrophoresis, Polyacrylamide Gel
  • Electrophoresis, Gel, Two-Dimensional
  • Developmental Biology
  • DNA Topoisomerases, Type II
 

Citation

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Cardenas, M. E., Dang, Q., Glover, C. V., & Gasser, S. M. (1992). Casein kinase II phosphorylates the eukaryote-specific C-terminal domain of topoisomerase II in vivo. EMBO J, 11(5), 1785–1796. https://doi.org/10.1002/j.1460-2075.1992.tb05230.x
Cardenas, M. E., Q. Dang, C. V. Glover, and S. M. Gasser. “Casein kinase II phosphorylates the eukaryote-specific C-terminal domain of topoisomerase II in vivo.EMBO J 11, no. 5 (May 1992): 1785–96. https://doi.org/10.1002/j.1460-2075.1992.tb05230.x.
Cardenas ME, Dang Q, Glover CV, Gasser SM. Casein kinase II phosphorylates the eukaryote-specific C-terminal domain of topoisomerase II in vivo. EMBO J. 1992 May;11(5):1785–96.
Cardenas, M. E., et al. “Casein kinase II phosphorylates the eukaryote-specific C-terminal domain of topoisomerase II in vivo.EMBO J, vol. 11, no. 5, May 1992, pp. 1785–96. Pubmed, doi:10.1002/j.1460-2075.1992.tb05230.x.
Cardenas ME, Dang Q, Glover CV, Gasser SM. Casein kinase II phosphorylates the eukaryote-specific C-terminal domain of topoisomerase II in vivo. EMBO J. 1992 May;11(5):1785–1796.

Published In

EMBO J

DOI

ISSN

0261-4189

Publication Date

May 1992

Volume

11

Issue

5

Start / End Page

1785 / 1796

Location

England

Related Subject Headings

  • Saccharomyces cerevisiae
  • Protein Serine-Threonine Kinases
  • Precipitin Tests
  • Phosphorylation
  • Peptide Mapping
  • Fluorescent Antibody Technique
  • Electrophoresis, Polyacrylamide Gel
  • Electrophoresis, Gel, Two-Dimensional
  • Developmental Biology
  • DNA Topoisomerases, Type II