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Oncostatin M enhances CCL21 expression by microvascular endothelial cells and increases the efficiency of dendritic cell trafficking to lymph nodes.

Publication ,  Journal Article
Sugaya, M; Fang, L; Cardones, AR; Kakinuma, T; Jaber, SH; Blauvelt, A; Hwang, ST
Published in: J Immunol
December 1, 2006

CCL21, a lymphatic endothelial cell (LEC)-derived chemokine, and its receptor CCR7 regulate dendritic cell (DC) trafficking to lymph nodes (LN), but it is unclear how CCL21 expression is regulated. Oncostatin M (OSM) is an IL-6-like cytokine synthesized by activated DC and other leukocytes. In vitro, OSM (but not TNF-alpha) stimulated CCL21 mRNA and protein expression by human dermal microvascular EC (DMEC) in an ERK1/2-dependent fashion. Conditioned medium from OSM-treated DMEC stimulated CCL21-dependent chemotaxis of mouse bone marrow-derived DC (BMDC). Cultured BMDC expressed OSM, which was increased with the addition of LPS. Topical application of the contact-sensitizing hapten, trinitrochlorobenzene, resulted in enhanced OSM expression in the skin, whereas cutaneous injection of TNF-alpha did not. Injection of OSM into the footpad increased CCL21 mRNA expression in the draining LN by approximately 10-fold and in mouse skin by approximately 4-fold without increasing CCR7 mRNA. In vitro, OSM increased the permeability of DMEC and lung microvascular EC monolayers to FITC-dextran beads, and, in vivo, it enhanced accumulation of Evans blue dye in draining LN by approximately 3-fold (p = 0.0291). Of note, OSM increased trafficking of BMDC injected in footpads to draining LN by 2-fold (p = 0.016). In summary, OSM up-regulates CCL21 expression in skin and draining regional LN. We propose that OSM is a regulator of CCL21 expression and endothelial permeability in skin, contributing to efficient migration of DC to regional LN.

Duke Scholars

Published In

J Immunol

DOI

ISSN

0022-1767

Publication Date

December 1, 2006

Volume

177

Issue

11

Start / End Page

7665 / 7672

Location

United States

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Skin
  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA, Messenger
  • Picryl Chloride
  • Oncostatin M
  • Mice, Inbred C57BL
  • Mice, Inbred BALB C
  • Mice
  • Lymph Nodes
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Sugaya, M., Fang, L., Cardones, A. R., Kakinuma, T., Jaber, S. H., Blauvelt, A., & Hwang, S. T. (2006). Oncostatin M enhances CCL21 expression by microvascular endothelial cells and increases the efficiency of dendritic cell trafficking to lymph nodes. J Immunol, 177(11), 7665–7672. https://doi.org/10.4049/jimmunol.177.11.7665
Sugaya, Makoto, Lei Fang, Adela R. Cardones, Takashi Kakinuma, Samer H. Jaber, Andrew Blauvelt, and Sam T. Hwang. “Oncostatin M enhances CCL21 expression by microvascular endothelial cells and increases the efficiency of dendritic cell trafficking to lymph nodes.J Immunol 177, no. 11 (December 1, 2006): 7665–72. https://doi.org/10.4049/jimmunol.177.11.7665.
Sugaya M, Fang L, Cardones AR, Kakinuma T, Jaber SH, Blauvelt A, et al. Oncostatin M enhances CCL21 expression by microvascular endothelial cells and increases the efficiency of dendritic cell trafficking to lymph nodes. J Immunol. 2006 Dec 1;177(11):7665–72.
Sugaya, Makoto, et al. “Oncostatin M enhances CCL21 expression by microvascular endothelial cells and increases the efficiency of dendritic cell trafficking to lymph nodes.J Immunol, vol. 177, no. 11, Dec. 2006, pp. 7665–72. Pubmed, doi:10.4049/jimmunol.177.11.7665.
Sugaya M, Fang L, Cardones AR, Kakinuma T, Jaber SH, Blauvelt A, Hwang ST. Oncostatin M enhances CCL21 expression by microvascular endothelial cells and increases the efficiency of dendritic cell trafficking to lymph nodes. J Immunol. 2006 Dec 1;177(11):7665–7672.

Published In

J Immunol

DOI

ISSN

0022-1767

Publication Date

December 1, 2006

Volume

177

Issue

11

Start / End Page

7665 / 7672

Location

United States

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Skin
  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA, Messenger
  • Picryl Chloride
  • Oncostatin M
  • Mice, Inbred C57BL
  • Mice, Inbred BALB C
  • Mice
  • Lymph Nodes