Characterization of conditioned place preference to cocaine in congenic dopamine transporter knockout female mice.

Published

Journal Article

RATIONALE: The dopamine transporter (DAT) is thought to play a major role in the rewarding effects of cocaine. Therefore, it is surprising that cocaine reveals conditioned effects in DAT knockout (DAT-KO) mice. OBJECTIVES: To examine these findings further, we obtained complete dose-effect curves for DAT-KO and DAT wild-type (DAT-WT) mice in a cocaine conditioned place preference (CPP) procedure. METHODS: Congenic C57BL6 background female DAT-KO and DAT-WT mice were conditioned in a three-compartment place preference apparatus. Conditioning consisted of three 30-min sessions with cocaine (2.5, 5.0, 10.0, 20.0, or 40.0 mg/kg) and three 30-min sessions with saline. The distribution of time in each choice compartment was determined after each pair of conditioning sessions (one cocaine and one saline session). RESULTS: DAT-WT mice revealed CPP over a wide range of cocaine doses (5.0-40 mg/kg), whereas DAT-KO mice revealed CPP over a more restricted range of doses, with consistent CPP only occurring with 10 mg/kg of cocaine. CONCLUSIONS: CPP for cocaine develops in both DAT-KO and DAT-WT mice; however, the dose range at which CPP develops is much more restricted in DAT-KO mice than in DAT-WT mice. These observations corroborate the significant role of DAT inhibition in cocaine's conditioned effects.

Full Text

Duke Authors

Cited Authors

  • Medvedev, IO; Gainetdinov, RR; Sotnikova, TD; Bohn, LM; Caron, MG; Dykstra, LA

Published Date

  • July 2005

Published In

Volume / Issue

  • 180 / 3

Start / End Page

  • 408 - 413

PubMed ID

  • 15719221

Pubmed Central ID

  • 15719221

International Standard Serial Number (ISSN)

  • 0033-3158

Digital Object Identifier (DOI)

  • 10.1007/s00213-005-2173-y

Language

  • eng

Conference Location

  • Germany