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Aromatic hydrocarbon receptor interaction with the retinoblastoma protein potentiates repression of E2F-dependent transcription and cell cycle arrest.

Publication ,  Journal Article
Puga, A; Barnes, SJ; Dalton, TP; Chang, CY; Knudsen, ES; Maier, MA
Published in: J Biol Chem
January 28, 2000

Polyhalogenated aromatic hydrocarbons, of which 2,3,7, 8-tetrachloro-p-dioxin (TCDD) is the prototype compound, elicit a variety of toxic, teratogenic, and carcinogenic responses in exposed animals and in humans. In cultured cells, TCDD shows marked effects on the regulation of cell cycle progression, including thymocyte apoptosis, induction of keratinocyte proliferation and terminal differentiation, and inhibition of estrogen-dependent proliferation in breast cancer cells. The presence of an LXCXE domain in the dioxin aromatic hydrocarbon receptor (AHR), suggested that the effects of TCDD on cell cycle regulation might be mediated by protein-protein interactions between AHR and the retinoblastoma protein (RB). Using the yeast two-hybrid system, AHR and RB were in fact shown to bind to each other. In vitro pull-down experiments with truncated AHR peptides indicated that at least two separate AHR domains form independent complexes with hypophosphorylated RB. Coimmunoprecipitation of whole cell lysates from human breast carcinoma MCF-7 cells, which express both proteins endogenously, revealed that AHR associates with RB in vivo only after receptor transformation and nuclear translocation. However, the AHR nuclear translocator and transcriptional heterodimerization partner, is not required for (nor is it a part of) the AHR.RB complexes detected in vitro. Ectopic expression of AHR and RB in human osteosarcoma SAOS-2 cells, which lack endogenous expression of both proteins, showed that AHR synergizes with RB to repress E2F-dependent transcription and to induce cell cycle arrest. Furthermore, AHR partly blocked T-antigen-mediated reversal of RB-dependent transcriptional repression. These results uncover a potential function for the AHR in cell cycle regulation and suggest that this function may be that of serving as an environmental sensor that signals cell cycle arrest when cells are exposed to certain environmental toxicants.

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Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

January 28, 2000

Volume

275

Issue

4

Start / End Page

2943 / 2950

Location

United States

Related Subject Headings

  • Two-Hybrid System Techniques
  • Tumor Cells, Cultured
  • Transcription, Genetic
  • Transcription Factors
  • Transcription Factor DP1
  • Retinoblastoma-Binding Protein 1
  • Retinoblastoma Protein
  • Repressor Proteins
  • Receptors, Aryl Hydrocarbon
  • Protein Binding
 

Citation

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Puga, A., Barnes, S. J., Dalton, T. P., Chang, C. Y., Knudsen, E. S., & Maier, M. A. (2000). Aromatic hydrocarbon receptor interaction with the retinoblastoma protein potentiates repression of E2F-dependent transcription and cell cycle arrest. J Biol Chem, 275(4), 2943–2950. https://doi.org/10.1074/jbc.275.4.2943
Puga, A., S. J. Barnes, T. P. Dalton, C. Y. Chang, E. S. Knudsen, and M. A. Maier. “Aromatic hydrocarbon receptor interaction with the retinoblastoma protein potentiates repression of E2F-dependent transcription and cell cycle arrest.J Biol Chem 275, no. 4 (January 28, 2000): 2943–50. https://doi.org/10.1074/jbc.275.4.2943.
Puga A, Barnes SJ, Dalton TP, Chang CY, Knudsen ES, Maier MA. Aromatic hydrocarbon receptor interaction with the retinoblastoma protein potentiates repression of E2F-dependent transcription and cell cycle arrest. J Biol Chem. 2000 Jan 28;275(4):2943–50.
Puga, A., et al. “Aromatic hydrocarbon receptor interaction with the retinoblastoma protein potentiates repression of E2F-dependent transcription and cell cycle arrest.J Biol Chem, vol. 275, no. 4, Jan. 2000, pp. 2943–50. Pubmed, doi:10.1074/jbc.275.4.2943.
Puga A, Barnes SJ, Dalton TP, Chang CY, Knudsen ES, Maier MA. Aromatic hydrocarbon receptor interaction with the retinoblastoma protein potentiates repression of E2F-dependent transcription and cell cycle arrest. J Biol Chem. 2000 Jan 28;275(4):2943–2950.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

January 28, 2000

Volume

275

Issue

4

Start / End Page

2943 / 2950

Location

United States

Related Subject Headings

  • Two-Hybrid System Techniques
  • Tumor Cells, Cultured
  • Transcription, Genetic
  • Transcription Factors
  • Transcription Factor DP1
  • Retinoblastoma-Binding Protein 1
  • Retinoblastoma Protein
  • Repressor Proteins
  • Receptors, Aryl Hydrocarbon
  • Protein Binding