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Endothelial progenitor cell infusion induces hematopoietic stem cell reconstitution in vivo.

Publication ,  Journal Article
Salter, AB; Meadows, SK; Muramoto, GG; Himburg, H; Doan, P; Daher, P; Russell, L; Chen, B; Chao, NJ; Chute, JP
Published in: Blood
February 26, 2009

Hematopoietic stem cells (HSCs) reside in association with bone marrow (BM) sinusoidal vessels in vivo, but the function of BM endothelial cells (ECs) in regulating hematopoiesis is unclear. We hypothesized that hematopoietic regeneration following injury is regulated by BM ECs. BALB/c mice were treated with total body irradiation (TBI) and then infused with C57Bl6-derived endothelial progenitor cells (EPCs) to augment endogenous BM EC activity. TBI caused pronounced disruption of the BM vasculature, BM hypocellularity, ablation of HSCs, and pancytopenia in control mice, whereas irradiated, EPC-treated mice displayed accelerated recovery of BM sinusoidal vessels, BM cellularity, peripheral blood white blood cells (WBCs), neutrophils, and platelets, and a 4.4-fold increase in BM HSCs. Systemic administration of anti-VE-cadherin antibody significantly delayed hematologic recovery in both EPC-treated mice and irradiated, non-EPC-treated mice compared with irradiated controls. These data demonstrate that allogeneic EPC infusions can augment hematopoiesis and suggest a relationship between BM microvascular recovery and hematopoietic reconstitution in vivo.

Duke Scholars

Published In

Blood

DOI

EISSN

1528-0020

Publication Date

February 26, 2009

Volume

113

Issue

9

Start / End Page

2104 / 2107

Location

United States

Related Subject Headings

  • Whole-Body Irradiation
  • Stem Cells
  • Stem Cell Transplantation
  • Recovery of Function
  • Mice, Inbred C57BL
  • Mice, Inbred BALB C
  • Mice
  • Immunology
  • Hematopoietic Stem Cells
  • Hematopoiesis
 

Citation

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Salter, A. B., Meadows, S. K., Muramoto, G. G., Himburg, H., Doan, P., Daher, P., … Chute, J. P. (2009). Endothelial progenitor cell infusion induces hematopoietic stem cell reconstitution in vivo. Blood, 113(9), 2104–2107. https://doi.org/10.1182/blood-2008-06-162941
Salter, Alice B., Sarah K. Meadows, Garrett G. Muramoto, Heather Himburg, Phuong Doan, Pamela Daher, Lauren Russell, Benny Chen, Nelson J. Chao, and John P. Chute. “Endothelial progenitor cell infusion induces hematopoietic stem cell reconstitution in vivo.Blood 113, no. 9 (February 26, 2009): 2104–7. https://doi.org/10.1182/blood-2008-06-162941.
Salter AB, Meadows SK, Muramoto GG, Himburg H, Doan P, Daher P, et al. Endothelial progenitor cell infusion induces hematopoietic stem cell reconstitution in vivo. Blood. 2009 Feb 26;113(9):2104–7.
Salter, Alice B., et al. “Endothelial progenitor cell infusion induces hematopoietic stem cell reconstitution in vivo.Blood, vol. 113, no. 9, Feb. 2009, pp. 2104–07. Pubmed, doi:10.1182/blood-2008-06-162941.
Salter AB, Meadows SK, Muramoto GG, Himburg H, Doan P, Daher P, Russell L, Chen B, Chao NJ, Chute JP. Endothelial progenitor cell infusion induces hematopoietic stem cell reconstitution in vivo. Blood. 2009 Feb 26;113(9):2104–2107.

Published In

Blood

DOI

EISSN

1528-0020

Publication Date

February 26, 2009

Volume

113

Issue

9

Start / End Page

2104 / 2107

Location

United States

Related Subject Headings

  • Whole-Body Irradiation
  • Stem Cells
  • Stem Cell Transplantation
  • Recovery of Function
  • Mice, Inbred C57BL
  • Mice, Inbred BALB C
  • Mice
  • Immunology
  • Hematopoietic Stem Cells
  • Hematopoiesis