Skip to main content

Molecular profile and partial functional analysis of novel endothelial cell-derived growth factors that regulate hematopoiesis.

Publication ,  Journal Article
Chute, JP; Muramoto, GG; Dressman, HK; Wolfe, G; Chao, NJ; Lin, S
Published in: Stem Cells
May 2006

Recent progress has been made in the identification of the osteoblastic cellular niche for hematopoietic stem cells (HSCs) within the bone marrow (BM). Attempts to identify the soluble factors that regulate HSC self-renewal have been less successful. We have demonstrated that primary human brain endothelial cells (HUBECs) support the ex vivo amplification of primitive human BM and cord blood cells capable of repopulating non-obese diabetic/severe combined immunodeficient repopulating (SCID) mice (SCID repopulating cells [SRCs]). In this study, we sought to characterize the soluble hematopoietic activity produced by HUBECs and to identify the growth factors secreted by HUBECs that contribute to this HSC-supportive effect. Extended noncontact HUBEC cultures supported an eight-fold increase in SRCs when combined with thrombopoietin, stem cell factor, and Flt-3 ligand compared with input CD34(+) cells or cytokines alone. Gene expression analysis of HUBEC biological replicates identified 65 differentially expressed, nonredundant transcripts without annotated hematopoietic activity. Gene ontology studies of the HUBEC transcriptome revealed a high concentration of genes encoding extracellular proteins with cell-cell signaling function. Functional analyses demonstrated that adrenomedullin, a vasodilatory hormone, synergized with stem cell factor and Flt-3 ligand to induce the proliferation of primitive human CD34(+)CD38(-)lin(-) cells and promoted the expansion of CD34(+) progenitors in culture. These data demonstrate the potential of primary HUBECs as a reservoir for the discovery of novel secreted proteins that regulate human hematopoiesis.

Duke Scholars

Published In

Stem Cells

DOI

ISSN

1066-5099

Publication Date

May 2006

Volume

24

Issue

5

Start / End Page

1315 / 1327

Location

England

Related Subject Headings

  • Vasodilator Agents
  • Solubility
  • Mice, SCID
  • Mice, Inbred NOD
  • Mice
  • Immunology
  • Humans
  • Hematopoietic Stem Cells
  • Hematopoietic Stem Cell Transplantation
  • Hematopoiesis
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Chute, J. P., Muramoto, G. G., Dressman, H. K., Wolfe, G., Chao, N. J., & Lin, S. (2006). Molecular profile and partial functional analysis of novel endothelial cell-derived growth factors that regulate hematopoiesis. Stem Cells, 24(5), 1315–1327. https://doi.org/10.1634/stemcells.2005-0029
Chute, John P., Garrett G. Muramoto, Holly K. Dressman, Gary Wolfe, Nelson J. Chao, and Simon Lin. “Molecular profile and partial functional analysis of novel endothelial cell-derived growth factors that regulate hematopoiesis.Stem Cells 24, no. 5 (May 2006): 1315–27. https://doi.org/10.1634/stemcells.2005-0029.
Chute JP, Muramoto GG, Dressman HK, Wolfe G, Chao NJ, Lin S. Molecular profile and partial functional analysis of novel endothelial cell-derived growth factors that regulate hematopoiesis. Stem Cells. 2006 May;24(5):1315–27.
Chute, John P., et al. “Molecular profile and partial functional analysis of novel endothelial cell-derived growth factors that regulate hematopoiesis.Stem Cells, vol. 24, no. 5, May 2006, pp. 1315–27. Pubmed, doi:10.1634/stemcells.2005-0029.
Chute JP, Muramoto GG, Dressman HK, Wolfe G, Chao NJ, Lin S. Molecular profile and partial functional analysis of novel endothelial cell-derived growth factors that regulate hematopoiesis. Stem Cells. 2006 May;24(5):1315–1327.

Published In

Stem Cells

DOI

ISSN

1066-5099

Publication Date

May 2006

Volume

24

Issue

5

Start / End Page

1315 / 1327

Location

England

Related Subject Headings

  • Vasodilator Agents
  • Solubility
  • Mice, SCID
  • Mice, Inbred NOD
  • Mice
  • Immunology
  • Humans
  • Hematopoietic Stem Cells
  • Hematopoietic Stem Cell Transplantation
  • Hematopoiesis