High-resolution in vivo imaging of blood vessels without labeling

Journal Article

We demonstrate that both oxyhemoglobin and deoxyhemoglobin have sequential two-color, two-photon absorption properties that can serve as endogenous contrasts in microvasculature imaging. Using a sensitive modulation transfer technique, we are able to image hemoglobin in red blood cells with micrometer resolution, both in vitro and in vivo. We show that excellent contrast from hemoglobin without any labeling can be obtained in tissue. © 2007 Optical Society of America.

Full Text

Duke Authors

Cited Authors

  • Fu, D; Ye, T; Matthews, TE; Chen, BJ; Yurtserver, G; Warren, WS

Published Date

  • 2007

Published In

Volume / Issue

  • 32 / 18

Start / End Page

  • 2641 - 2643

PubMed ID

  • 17873920

International Standard Serial Number (ISSN)

  • 0146-9592

Digital Object Identifier (DOI)

  • 10.1364/OL.32.002641