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Interleukin-17 synergizes with IFNγ or TNFα to promote inflammatory mediator release and intercellular adhesion molecule-1 (ICAM-1) expression in human intervertebral disc cells.

Publication ,  Journal Article
Gabr, MA; Jing, L; Helbling, AR; Sinclair, SM; Allen, KD; Shamji, MF; Richardson, WJ; Fitch, RD; Setton, LA; Chen, J
Published in: J Orthop Res
January 2011

Interleukin-17 (IL-17) is a cytokine recently shown to be elevated, along with interferon-γ (IFNγ) and tumor necrosis factor (TNFα), in degenerated and herniated intervertebral disc (IVD) tissues, suggesting a role for these cytokines in intervertebral disc disease. The objective of our study was to investigate the involvement of IL-17 and costimulants IFNγ and TNFα in intervertebral disc pathology. Cells were isolated from anulus fibrosus and nucleus pulposus tissues of patients undergoing surgery for intervertebral disc degeneration or scoliosis. The production of inflammatory mediators, nitric oxide (NOx), prostaglandin E2 (PGE2) and interleukin-6 (IL-6), as well as intercellular adhesion molecule (ICAM-1) expression, were quantified for cultured cells following exposure to IL-17, IFNγ, and TNFα. Intervertebral disc cells exposed to IL-17, IFNγ, or TNFα showed a remarkable increase in inflammatory mediator release and ICAM-1 expression (GLM and ANOVA, p < 0.05). Addition of IFNγ or TNFα to IL-17 demonstrated a synergistic increase in inflammatory mediator release, and a marked increase in ICAM-1 expression. These findings suggest that IVD cells not only respond with a catabolic phenotype to IL-17 and costimulants IFNγ and TNFα, but also express surface ligands with consequent potential to recruit additional lymphocytes and immune cells to the IVD microenvironment. IL-17 may be an important regulator of inflammation in the IVD pathologies.

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Published In

J Orthop Res

DOI

EISSN

1554-527X

Publication Date

January 2011

Volume

29

Issue

1

Start / End Page

1 / 7

Location

United States

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Orthopedics
  • Middle Aged
  • Male
  • Intervertebral Disc Displacement
  • Intervertebral Disc Degeneration
  • Intervertebral Disc
  • Interleukin-17
  • Interferon-gamma
  • Intercellular Adhesion Molecule-1
 

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Gabr, M. A., Jing, L., Helbling, A. R., Sinclair, S. M., Allen, K. D., Shamji, M. F., … Chen, J. (2011). Interleukin-17 synergizes with IFNγ or TNFα to promote inflammatory mediator release and intercellular adhesion molecule-1 (ICAM-1) expression in human intervertebral disc cells. J Orthop Res, 29(1), 1–7. https://doi.org/10.1002/jor.21206
Gabr, Mostafa A., Liufang Jing, Antonia R. Helbling, S Michael Sinclair, Kyle D. Allen, Mohammed F. Shamji, William J. Richardson, Robert D. Fitch, Lori A. Setton, and Jun Chen. “Interleukin-17 synergizes with IFNγ or TNFα to promote inflammatory mediator release and intercellular adhesion molecule-1 (ICAM-1) expression in human intervertebral disc cells.J Orthop Res 29, no. 1 (January 2011): 1–7. https://doi.org/10.1002/jor.21206.
Gabr MA, Jing L, Helbling AR, Sinclair SM, Allen KD, Shamji MF, Richardson WJ, Fitch RD, Setton LA, Chen J. Interleukin-17 synergizes with IFNγ or TNFα to promote inflammatory mediator release and intercellular adhesion molecule-1 (ICAM-1) expression in human intervertebral disc cells. J Orthop Res. 2011 Jan;29(1):1–7.
Journal cover image

Published In

J Orthop Res

DOI

EISSN

1554-527X

Publication Date

January 2011

Volume

29

Issue

1

Start / End Page

1 / 7

Location

United States

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Orthopedics
  • Middle Aged
  • Male
  • Intervertebral Disc Displacement
  • Intervertebral Disc Degeneration
  • Intervertebral Disc
  • Interleukin-17
  • Interferon-gamma
  • Intercellular Adhesion Molecule-1