Isomeric oligosaccharide analysis using hydrophilic interaction LC-ESI-MS/MS


Journal Article

A hydrophilic interaction liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS/MS) method was established for the separation and analysis of hexose tetrasaccharide linkage isomers in urine, plasma and blood spots. Using the LC-ESI-MS/MS method, the extent of interference from other isomers was also determined. It was found that the tentative identification of isomer 1 as maltotetraose was supported by the increase in both isomer 1 and Glc 4 observed in the plasma of GSD II patients, suggesting it to be derived from glycogen. It was also found that isomer 1 is the major isomer present in blood spot extracts and is probably derived from glycogen stored in the red cells.

Duke Authors

Cited Authors

  • Young, SP; An, Y; Van Hove, J; Stevens, RD; Chen, YT; Millington, DS

Published Date

  • December 1, 2002

Published In

  • Proceedings 50th Asms Conference on Mass Spectrometry and Allied Topics

Start / End Page

  • 413 - 414

Citation Source

  • Scopus