Purification of recombinant human precursor acid alpha-glucosidase.

Published

Journal Article

Large quantities of recombinant acid alpha-glucosidase are needed for in vivo experimentation of enzyme replacement therapy in Pompe disease. We describe a new purification method for the purification of this recombinant enzyme from tissue culture medium consisting of concanavalin A affinity chromatography, hydrophobic interaction chromatography, affinity chromatography on Superdex, and anion exchange chromatography. The new method is amenable to scale up, and has increased speed, and improved reproducibility with similar high yield and purification efficiency when compared to previous methods.

Full Text

Duke Authors

Cited Authors

  • Van Hove, JL; Yang, HW; Oliver, LM; Pennybacker, MF; Chen, YT

Published Date

  • October 1997

Published In

Volume / Issue

  • 43 / 3

Start / End Page

  • 613 - 623

PubMed ID

  • 9352080

Pubmed Central ID

  • 9352080

International Standard Serial Number (ISSN)

  • 1039-9712

Language

  • eng

Conference Location

  • England