Purification of recombinant human precursor acid alpha-glucosidase.
Publication
, Journal Article
Van Hove, JL; Yang, HW; Oliver, LM; Pennybacker, MF; Chen, YT
Published in: Biochem Mol Biol Int
October 1997
Large quantities of recombinant acid alpha-glucosidase are needed for in vivo experimentation of enzyme replacement therapy in Pompe disease. We describe a new purification method for the purification of this recombinant enzyme from tissue culture medium consisting of concanavalin A affinity chromatography, hydrophobic interaction chromatography, affinity chromatography on Superdex, and anion exchange chromatography. The new method is amenable to scale up, and has increased speed, and improved reproducibility with similar high yield and purification efficiency when compared to previous methods.
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Published In
Biochem Mol Biol Int
DOI
ISSN
1039-9712
Publication Date
October 1997
Volume
43
Issue
3
Start / End Page
613 / 623
Location
England
Related Subject Headings
- alpha-Glucosidases
- Humans
- Glycogen Storage Disease Type II
- Fibroblasts
- Cricetinae
- Chromatography
- CHO Cells
- Biochemistry & Molecular Biology
- Animals
- 1101 Medical Biochemistry and Metabolomics
Citation
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Chicago
ICMJE
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NLM
Van Hove, J. L., Yang, H. W., Oliver, L. M., Pennybacker, M. F., & Chen, Y. T. (1997). Purification of recombinant human precursor acid alpha-glucosidase. Biochem Mol Biol Int, 43(3), 613–623. https://doi.org/10.1080/15216549700204421
Van Hove, J. L., H. W. Yang, L. M. Oliver, M. F. Pennybacker, and Y. T. Chen. “Purification of recombinant human precursor acid alpha-glucosidase.” Biochem Mol Biol Int 43, no. 3 (October 1997): 613–23. https://doi.org/10.1080/15216549700204421.
Van Hove JL, Yang HW, Oliver LM, Pennybacker MF, Chen YT. Purification of recombinant human precursor acid alpha-glucosidase. Biochem Mol Biol Int. 1997 Oct;43(3):613–23.
Van Hove, J. L., et al. “Purification of recombinant human precursor acid alpha-glucosidase.” Biochem Mol Biol Int, vol. 43, no. 3, Oct. 1997, pp. 613–23. Pubmed, doi:10.1080/15216549700204421.
Van Hove JL, Yang HW, Oliver LM, Pennybacker MF, Chen YT. Purification of recombinant human precursor acid alpha-glucosidase. Biochem Mol Biol Int. 1997 Oct;43(3):613–623.
Published In
Biochem Mol Biol Int
DOI
ISSN
1039-9712
Publication Date
October 1997
Volume
43
Issue
3
Start / End Page
613 / 623
Location
England
Related Subject Headings
- alpha-Glucosidases
- Humans
- Glycogen Storage Disease Type II
- Fibroblasts
- Cricetinae
- Chromatography
- CHO Cells
- Biochemistry & Molecular Biology
- Animals
- 1101 Medical Biochemistry and Metabolomics