Polysome profiling of the malaria parasite Plasmodium falciparum.

Journal Article

In the malaria parasite Plasmodium falciparum, global studies of translational regulation have been hampered by the inability to isolate malaria polysomes. We describe here a novel method for polysome profiling in P. falciparum, a powerful approach which allows both a global view of translation and the measurement of ribosomal loading and density for specific mRNAs. Simultaneous lysis of infected erythrocytes and parasites releases stable, intact malaria polysomes, which are then purified by centrifugation through a sucrose cushion. The polysomes are resuspended, separated by velocity sedimentation and then fractionated, yielding a characteristic polysome profile reflecting the global level of translational activity in the parasite. RNA isolated from specific fractions can be used to determine the density of ribosomes loaded onto a particular transcript of interest, and is free of host ribosome contamination. Thus, our approach opens translational regulation in malaria to genome-wide analysis.

Full Text

Duke Authors

Cited Authors

  • Lacsina, JR; LaMonte, G; Nicchitta, CV; Chi, J-T

Published Date

  • September 2011

Published In

Volume / Issue

  • 179 / 1

Start / End Page

  • 42 - 46

PubMed ID

  • 21605599

Electronic International Standard Serial Number (EISSN)

  • 1872-9428

Digital Object Identifier (DOI)

  • 10.1016/j.molbiopara.2011.05.003

Language

  • eng

Conference Location

  • Netherlands