Genomewide view of gene silencing by small interfering RNAs.
Published
Journal Article
RNA interference (RNAi) is an evolutionarily conserved mechanism in plant and animal cells that directs the degradation of messenger RNAs homologous to short double-stranded RNAs termed small interfering RNA (siRNA). The ability of siRNA to direct gene silencing in mammalian cells has raised the possibility that siRNA might be used to investigate gene function in a high throughput fashion or to modulate gene expression in human diseases. The specificity of siRNA-mediated silencing, a critical consideration in these applications, has not been addressed on a genomewide scale. Here we show that siRNA-induced gene silencing of transient or stably expressed mRNA is highly gene-specific and does not produce secondary effects detectable by genomewide expression profiling. A test for transitive RNAi, extension of the RNAi effect to sequences 5' of the target region that has been observed in Caenorhabditis elegans, was unable to detect this phenomenon in human cells.
Full Text
Duke Authors
Cited Authors
- Chi, J-T; Chang, HY; Wang, NN; Chang, DS; Dunphy, N; Brown, PO
Published Date
- May 27, 2003
Published In
Volume / Issue
- 100 / 11
Start / End Page
- 6343 - 6346
PubMed ID
- 12730368
Pubmed Central ID
- 12730368
International Standard Serial Number (ISSN)
- 0027-8424
Digital Object Identifier (DOI)
- 10.1073/pnas.1037853100
Language
- eng
Conference Location
- United States