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Systemic and cell type-specific gene expression patterns in scleroderma skin.

Publication ,  Journal Article
Whitfield, ML; Finlay, DR; Murray, JI; Troyanskaya, OG; Chi, J-T; Pergamenschikov, A; McCalmont, TH; Brown, PO; Botstein, D; Connolly, MK
Published in: Proc Natl Acad Sci U S A
October 14, 2003

We used DNA microarrays representing >12,000 human genes to characterize gene expression patterns in skin biopsies from individuals with a diagnosis of systemic sclerosis with diffuse scleroderma. We found consistent differences in the patterns of gene expression between skin biopsies from individuals with scleroderma and those from normal, unaffected individuals. The biopsies from affected individuals showed nearly indistinguishable patterns of gene expression in clinically affected and clinically unaffected tissue, even though these were clearly distinguishable from the patterns found in similar tissue from unaffected individuals. Genes characteristically expressed in endothelial cells, B lymphocytes, and fibroblasts showed differential expression between scleroderma and normal biopsies. Analysis of lymphocyte populations in scleroderma skin biopsies by immunohistochemistry suggest the B lymphocyte signature observed on our arrays is from CD20+ B cells. These results provide evidence that scleroderma has systemic manifestations that affect multiple cell types and suggests genes that could be used as potential markers for the disease.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

October 14, 2003

Volume

100

Issue

21

Start / End Page

12319 / 12324

Location

United States

Related Subject Headings

  • Skin
  • Scleroderma, Diffuse
  • Middle Aged
  • Male
  • Humans
  • Gene Expression Profiling
  • Female
  • Case-Control Studies
  • B-Lymphocyte Subsets
  • Antigens, CD20
 

Citation

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MLA
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Whitfield, M. L., Finlay, D. R., Murray, J. I., Troyanskaya, O. G., Chi, J.-T., Pergamenschikov, A., … Connolly, M. K. (2003). Systemic and cell type-specific gene expression patterns in scleroderma skin. Proc Natl Acad Sci U S A, 100(21), 12319–12324. https://doi.org/10.1073/pnas.1635114100
Whitfield, Michael L., Deborah R. Finlay, John Isaac Murray, Olga G. Troyanskaya, Jen-Tsan Chi, Alexander Pergamenschikov, Timothy H. McCalmont, Patrick O. Brown, David Botstein, and M Kari Connolly. “Systemic and cell type-specific gene expression patterns in scleroderma skin.Proc Natl Acad Sci U S A 100, no. 21 (October 14, 2003): 12319–24. https://doi.org/10.1073/pnas.1635114100.
Whitfield ML, Finlay DR, Murray JI, Troyanskaya OG, Chi J-T, Pergamenschikov A, et al. Systemic and cell type-specific gene expression patterns in scleroderma skin. Proc Natl Acad Sci U S A. 2003 Oct 14;100(21):12319–24.
Whitfield, Michael L., et al. “Systemic and cell type-specific gene expression patterns in scleroderma skin.Proc Natl Acad Sci U S A, vol. 100, no. 21, Oct. 2003, pp. 12319–24. Pubmed, doi:10.1073/pnas.1635114100.
Whitfield ML, Finlay DR, Murray JI, Troyanskaya OG, Chi J-T, Pergamenschikov A, McCalmont TH, Brown PO, Botstein D, Connolly MK. Systemic and cell type-specific gene expression patterns in scleroderma skin. Proc Natl Acad Sci U S A. 2003 Oct 14;100(21):12319–12324.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

October 14, 2003

Volume

100

Issue

21

Start / End Page

12319 / 12324

Location

United States

Related Subject Headings

  • Skin
  • Scleroderma, Diffuse
  • Middle Aged
  • Male
  • Humans
  • Gene Expression Profiling
  • Female
  • Case-Control Studies
  • B-Lymphocyte Subsets
  • Antigens, CD20