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A novel basal promoter element is required for expression of the rat tyrosine hydroxylase gene.

Publication ,  Journal Article
Patankar, S; Lazaroff, M; Yoon, SO; Chikaraishi, DM
Published in: J Neurosci
June 1, 1997

Transcription of the rat tyrosine hydroxylase (TH) gene is controlled by enhancer sequences in its 5' flanking region; these enhancers include the AP1, dyad, and cAMP response element (CRE) motifs. We show that a novel basal promoter element (-17 GCCTGCCTGGCGA -5) positioned between the TATA box and +1 works in conjunction with the upstream AP1-dyad and CRE enhancers but cannot support transcription by itself. A mutation of this element, termed partial dyad, reduces basal expression of a reporter gene in TH-positive cell lines and TH-negative lines but has no effect on cAMP- or KCl-induced expression. A double mutant at positions -17 and -11 of the partial dyad reduces transcriptional activation by 80%. Conversely, insertion of this element into a heterologous promoter restores basal expression to levels mediated by the native TH promoter. The partial dyad is a novel activational element that is required for full expression of the TH gene and may assist in the function of the AP1, dyad, and CRE motifs and also other enhancers further upstream. Hence, the rat TH gene is unusual in that its enhancers will not function with a heterologous promoter but require a specific TH promoter sequence for full activation.

Duke Scholars

Published In

J Neurosci

DOI

ISSN

0270-6474

Publication Date

June 1, 1997

Volume

17

Issue

11

Start / End Page

4076 / 4086

Location

United States

Related Subject Headings

  • Tyrosine 3-Monooxygenase
  • Transcription, Genetic
  • Transcription Factor AP-1
  • TATA Box
  • Rats
  • PC12 Cells
  • Neurology & Neurosurgery
  • Neuroblastoma
  • Molecular Sequence Data
  • Mice
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Patankar, S., Lazaroff, M., Yoon, S. O., & Chikaraishi, D. M. (1997). A novel basal promoter element is required for expression of the rat tyrosine hydroxylase gene. J Neurosci, 17(11), 4076–4086. https://doi.org/10.1523/JNEUROSCI.17-11-04076.1997
Patankar, S., M. Lazaroff, S. O. Yoon, and D. M. Chikaraishi. “A novel basal promoter element is required for expression of the rat tyrosine hydroxylase gene.J Neurosci 17, no. 11 (June 1, 1997): 4076–86. https://doi.org/10.1523/JNEUROSCI.17-11-04076.1997.
Patankar S, Lazaroff M, Yoon SO, Chikaraishi DM. A novel basal promoter element is required for expression of the rat tyrosine hydroxylase gene. J Neurosci. 1997 Jun 1;17(11):4076–86.
Patankar, S., et al. “A novel basal promoter element is required for expression of the rat tyrosine hydroxylase gene.J Neurosci, vol. 17, no. 11, June 1997, pp. 4076–86. Pubmed, doi:10.1523/JNEUROSCI.17-11-04076.1997.
Patankar S, Lazaroff M, Yoon SO, Chikaraishi DM. A novel basal promoter element is required for expression of the rat tyrosine hydroxylase gene. J Neurosci. 1997 Jun 1;17(11):4076–4086.

Published In

J Neurosci

DOI

ISSN

0270-6474

Publication Date

June 1, 1997

Volume

17

Issue

11

Start / End Page

4076 / 4086

Location

United States

Related Subject Headings

  • Tyrosine 3-Monooxygenase
  • Transcription, Genetic
  • Transcription Factor AP-1
  • TATA Box
  • Rats
  • PC12 Cells
  • Neurology & Neurosurgery
  • Neuroblastoma
  • Molecular Sequence Data
  • Mice