DNA regulatory sequences of the rat tyrosine hydroxylase gene direct correct catecholaminergic cell-type specificity of a human growth hormone reporter in the CNS of transgenic mice causing a dwarf phenotype.

Published

Journal Article

Transgenic mice bearing 4.8 kilobases (kb) of upstream rat tyrosine hydroxylase (TH) sequences linked to a human growth hormone gene (hGH) exhibited cell-specific expression of hGH in all the appropriate catecholaminergic neurons in the central nervous system (CNS), although with different penetrance in two different mouse lineages. No ectopic expression was observed in any brain or peripheral region in one founder and its progeny. In another founder there was some ectopic expression in addition to appropriate and high levels of tissue-specific expression in all catecholaminergic areas. These results identify regulatory sequences that are sufficient for targeting expression to all catecholaminergic CNS neurons. Also, expression of exogenous hGH in the hypothalamus caused a dwarf phenotype, generating a novel genetic model for GH deficiency of hypothalamic origin.

Full Text

Duke Authors

Cited Authors

  • Banerjee, SA; Roffler-Tarlov, S; Szabo, M; Frohman, L; Chikaraishi, DM

Published Date

  • July 1994

Published In

Volume / Issue

  • 24 / 1-4

Start / End Page

  • 89 - 106

PubMed ID

  • 7968381

Pubmed Central ID

  • 7968381

International Standard Serial Number (ISSN)

  • 0169-328X

Digital Object Identifier (DOI)

  • 10.1016/0169-328x(94)90121-x

Language

  • eng

Conference Location

  • Netherlands