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Regulation of neurogenesis and neuronal differentiation in primary and immortalized cells from mouse olfactory epithelium.

Publication ,  Journal Article
Calof, AL; Lander, AD; Chikaraishi, DM
Published in: Ciba Found Symp
1991

We have developed an in vitro system for studying molecular events regulating neurogenesis in the mouse olfactory epithelium (OE). Our observations suggest that two types of neuronal precursor may be involved: (1) a transiently existing, immediate neuronal precursor (INP), which generates two postmitotic daughter neurons; and (2) a neuroepithelial stem cell, which may be the basal cell (or some subclass of basal cell) of the OE, and is presumed to be the progenitor of the INP. Using antibody markers that distinguish basal cells and postmitotic receptor neurons in vitro and in vivo, we have shown that neurogenesis occurs early on in OE cultures, but then ceases because INPs divide only once to generate postmitotic neurons and no new INPs are produced by basal cells. To determine whether the basal cell-to-INP transition, or proliferation and neuronal differentiation of the INP, are regulated by crucial growth factors or cellular interactions, we are testing various polypeptide growth factors and extracellular matrix proteins for their effects on OE neurogenesis in vitro. We have also generated immortalized OE cell lines by using retroviruses to transduce oncogenes into cultured OE cells. One such cell line (derived from a primary OE basal cell culture) develops branching processes when transplanted into neonatal mouse brain--a condition in which cells from freshly isolated OE can undergo apparent morphological differentiation into neurons.

Duke Scholars

Published In

Ciba Found Symp

DOI

ISSN

0300-5208

Publication Date

1991

Volume

160

Start / End Page

249 / 265

Location

Netherlands

Related Subject Headings

  • Sensory Receptor Cells
  • Olfactory Mucosa
  • Mice
  • Epithelium
  • Epithelial Cells
  • Culture Techniques
  • Cell Line
  • Cell Differentiation
  • Cell Communication
  • Animals
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Calof, A. L., Lander, A. D., & Chikaraishi, D. M. (1991). Regulation of neurogenesis and neuronal differentiation in primary and immortalized cells from mouse olfactory epithelium. Ciba Found Symp, 160, 249–265. https://doi.org/10.1002/9780470514122.ch13
Calof, A. L., A. D. Lander, and D. M. Chikaraishi. “Regulation of neurogenesis and neuronal differentiation in primary and immortalized cells from mouse olfactory epithelium.Ciba Found Symp 160 (1991): 249–65. https://doi.org/10.1002/9780470514122.ch13.
Calof, A. L., et al. “Regulation of neurogenesis and neuronal differentiation in primary and immortalized cells from mouse olfactory epithelium.Ciba Found Symp, vol. 160, 1991, pp. 249–65. Pubmed, doi:10.1002/9780470514122.ch13.

Published In

Ciba Found Symp

DOI

ISSN

0300-5208

Publication Date

1991

Volume

160

Start / End Page

249 / 265

Location

Netherlands

Related Subject Headings

  • Sensory Receptor Cells
  • Olfactory Mucosa
  • Mice
  • Epithelium
  • Epithelial Cells
  • Culture Techniques
  • Cell Line
  • Cell Differentiation
  • Cell Communication
  • Animals