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Identification and cell type specificity of the tyrosine hydroxylase gene promoter.

Publication ,  Journal Article
Harrington, CA; Lewis, EJ; Krzemien, D; Chikaraishi, DM
Published in: Nucleic Acids Res
March 11, 1987

Genomic DNA encoding the rat tyrosine hydroxylase (TH) gene was isolated from a lambda phage library using a nick-translated fragment from a cDNA clone for rat TH. We have determined the initiation site for TH RNA synthesis and have sequenced 1100 bases of the primary transcript and 5' flanking region. The 5' end of the transcript is the same in several rat tissues in which TH is expressed as well as in rat pheochromocytoma cells (PC). RNA prepared from PC cells that had been stimulated with dexamethasone also mapped to the same transcription start site. Sequence upstream from the initiation site contains the canonical TATA box, but no apparent CAAT box. When a portion of the 5' flanking region of the TH gene (-773 to + 27) is fused to the chloramphenicol acetyltransferase (CAT) gene, it promotes expression of CAT in pheochromocytoma cells and GH4 cells, but not in two neural tumour lines, RT4-D and B103, nor in several non neural cell lines. This suggests that this region of the TH gene has features that confer tissue-restricted expression on the TH promoter.

Duke Scholars

Published In

Nucleic Acids Res

DOI

ISSN

0305-1048

Publication Date

March 11, 1987

Volume

15

Issue

5

Start / End Page

2363 / 2384

Location

England

Related Subject Headings

  • Tyrosine 3-Monooxygenase
  • Rats
  • Protein Biosynthesis
  • Promoter Regions, Genetic
  • Plasmids
  • Pheochromocytoma
  • Nucleic Acid Hybridization
  • Genes
  • Developmental Biology
  • DNA Restriction Enzymes
 

Citation

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MLA
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Harrington, C. A., Lewis, E. J., Krzemien, D., & Chikaraishi, D. M. (1987). Identification and cell type specificity of the tyrosine hydroxylase gene promoter. Nucleic Acids Res, 15(5), 2363–2384. https://doi.org/10.1093/nar/15.5.2363
Harrington, C. A., E. J. Lewis, D. Krzemien, and D. M. Chikaraishi. “Identification and cell type specificity of the tyrosine hydroxylase gene promoter.Nucleic Acids Res 15, no. 5 (March 11, 1987): 2363–84. https://doi.org/10.1093/nar/15.5.2363.
Harrington CA, Lewis EJ, Krzemien D, Chikaraishi DM. Identification and cell type specificity of the tyrosine hydroxylase gene promoter. Nucleic Acids Res. 1987 Mar 11;15(5):2363–84.
Harrington, C. A., et al. “Identification and cell type specificity of the tyrosine hydroxylase gene promoter.Nucleic Acids Res, vol. 15, no. 5, Mar. 1987, pp. 2363–84. Pubmed, doi:10.1093/nar/15.5.2363.
Harrington CA, Lewis EJ, Krzemien D, Chikaraishi DM. Identification and cell type specificity of the tyrosine hydroxylase gene promoter. Nucleic Acids Res. 1987 Mar 11;15(5):2363–2384.
Journal cover image

Published In

Nucleic Acids Res

DOI

ISSN

0305-1048

Publication Date

March 11, 1987

Volume

15

Issue

5

Start / End Page

2363 / 2384

Location

England

Related Subject Headings

  • Tyrosine 3-Monooxygenase
  • Rats
  • Protein Biosynthesis
  • Promoter Regions, Genetic
  • Plasmids
  • Pheochromocytoma
  • Nucleic Acid Hybridization
  • Genes
  • Developmental Biology
  • DNA Restriction Enzymes