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Cloning of DNA corresponding to rare transcripts of rat brain: evidence of transcriptional and post-transcriptional control and of the existence of nonpolyadenylated transcripts.

Publication ,  Journal Article
Brilliant, MH; Sueoka, N; Chikaraishi, DM
Published in: Mol Cell Biol
October 1984

To examine the expression of genes encoding rare transcripts in the rat brain, we have characterized genomic DNA clones corresponding to this class. In brain cells, as in all cell types, rare transcripts constitute the majority of different sequences transcribed. Moreover, when compared with other tissues or cultured cells, brain tissue may be expected to have an even larger set of rare transcripts, some of which could be restricted to subpopulations of neural cells. We have identified seven clones whose transcripts are nonabundant, averaging less than three copies per cell. Clone rg13 (rat genomic 13) RNA was detected only in the brain, whereas RNA of a second clone, rg40, was also detected in the brain and in a melanoma. Transcripts of rg13 were found in cerebellum, cerebral cortex, and regions underlying the cortex, whereas rg40 transcripts were not detected in the cerebellum. Transcripts of both rg13 and rg40 were found in pelleted polysomal RNA. RNA of another clone, rg34, was found in the brain, liver, and kidney but was found in pelleted polysomal RNA only in the brain, suggesting that its expression may be post-transcriptionally controlled. The remaining four clones represent rare transcripts that are common to the brain, liver, and kidney; rg18 RNA is restricted to the nucleus, whereas rg3, rg26, and rg36 transcripts are found in the cytoplasm of all three tissues. Transcripts of the brain-specific clone, rg13, and the commonly expressed clone, rg3, are nonpolyadenylated, presumably belonging to the high-complexity, nonpolyadenylated class of transcripts in the mammalian brain.

Duke Scholars

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

October 1984

Volume

4

Issue

10

Start / End Page

2187 / 2197

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Tissue Distribution
  • Rats
  • RNA, Neoplasm
  • RNA, Messenger
  • RNA
  • Protein Processing, Post-Translational
  • Poly A
  • Liver
  • Kidney
 

Citation

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MLA
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Brilliant, M. H., Sueoka, N., & Chikaraishi, D. M. (1984). Cloning of DNA corresponding to rare transcripts of rat brain: evidence of transcriptional and post-transcriptional control and of the existence of nonpolyadenylated transcripts. Mol Cell Biol, 4(10), 2187–2197. https://doi.org/10.1128/mcb.4.10.2187-2197.1984
Brilliant, M. H., N. Sueoka, and D. M. Chikaraishi. “Cloning of DNA corresponding to rare transcripts of rat brain: evidence of transcriptional and post-transcriptional control and of the existence of nonpolyadenylated transcripts.Mol Cell Biol 4, no. 10 (October 1984): 2187–97. https://doi.org/10.1128/mcb.4.10.2187-2197.1984.
Brilliant, M. H., et al. “Cloning of DNA corresponding to rare transcripts of rat brain: evidence of transcriptional and post-transcriptional control and of the existence of nonpolyadenylated transcripts.Mol Cell Biol, vol. 4, no. 10, Oct. 1984, pp. 2187–97. Pubmed, doi:10.1128/mcb.4.10.2187-2197.1984.

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

October 1984

Volume

4

Issue

10

Start / End Page

2187 / 2197

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Tissue Distribution
  • Rats
  • RNA, Neoplasm
  • RNA, Messenger
  • RNA
  • Protein Processing, Post-Translational
  • Poly A
  • Liver
  • Kidney