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Genomic organization of rat rDNA.

Publication ,  Journal Article
Chikaraishi, DM; Buchanan, L; Danna, KJ; Harrington, CA
Published in: Nucleic Acids Res
September 24, 1983

A detailed restriction map was determined for a 10.9 KB region that contains the initiation site for 45S pre-rRNA and the first 1.7 KB of the 18S rRNA coding region. When the restriction pattern of the cloned rDNA was compared with that of total rat DNA, the rDNA regions of both Sprague-Dawley and BD-9 rats were identical to each other and to that of the cloned rDNA. However, both strains exhibit a major polymorphism consisting of an insertion of 0.9 KB of DNA in the nontranscribed spacer between 0.29 KB and 1.8 KB upstream from the 45S RNA initiation site. This region consists of tandem repeats approximately 130 base pairs in length. These repeats contain large poly T tracts and are similar in sequence to analogous elements 5' to the origin of mouse rRNA transcription. Regions containing highly repetitious DNA sequences were located at sites 2.8 KB and 4.3 KB upstream from the initiation site. The repetitive sequence at 2.8 KB from the initiation site anneal to a known Alu-equivalent type 2 sequence derived from the second intron of the rat growth hormone gene.

Duke Scholars

Published In

Nucleic Acids Res

DOI

ISSN

0305-1048

Publication Date

September 24, 1983

Volume

11

Issue

18

Start / End Page

6437 / 6452

Location

England

Related Subject Headings

  • Species Specificity
  • Rats, Inbred Strains
  • Rats
  • RNA, Ribosomal
  • Polymorphism, Genetic
  • Plasmids
  • Nucleic Acid Hybridization
  • Genes
  • Developmental Biology
  • DNA, Ribosomal
 

Citation

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Chikaraishi, D. M., Buchanan, L., Danna, K. J., & Harrington, C. A. (1983). Genomic organization of rat rDNA. Nucleic Acids Res, 11(18), 6437–6452. https://doi.org/10.1093/nar/11.18.6437
Chikaraishi, D. M., L. Buchanan, K. J. Danna, and C. A. Harrington. “Genomic organization of rat rDNA.Nucleic Acids Res 11, no. 18 (September 24, 1983): 6437–52. https://doi.org/10.1093/nar/11.18.6437.
Chikaraishi DM, Buchanan L, Danna KJ, Harrington CA. Genomic organization of rat rDNA. Nucleic Acids Res. 1983 Sep 24;11(18):6437–52.
Chikaraishi, D. M., et al. “Genomic organization of rat rDNA.Nucleic Acids Res, vol. 11, no. 18, Sept. 1983, pp. 6437–52. Pubmed, doi:10.1093/nar/11.18.6437.
Chikaraishi DM, Buchanan L, Danna KJ, Harrington CA. Genomic organization of rat rDNA. Nucleic Acids Res. 1983 Sep 24;11(18):6437–6452.
Journal cover image

Published In

Nucleic Acids Res

DOI

ISSN

0305-1048

Publication Date

September 24, 1983

Volume

11

Issue

18

Start / End Page

6437 / 6452

Location

England

Related Subject Headings

  • Species Specificity
  • Rats, Inbred Strains
  • Rats
  • RNA, Ribosomal
  • Polymorphism, Genetic
  • Plasmids
  • Nucleic Acid Hybridization
  • Genes
  • Developmental Biology
  • DNA, Ribosomal