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Simian virus 40 transcriptional complexes incorporate mercurated nucleotides into RNA in vitro.

Publication ,  Journal Article
Chikaraishi, DM; Danna, KJ
Published in: J Virol
January 1981

Simian virus 40 Sarkosyl transcriptional complexes incorporated mercurated nucleotide precursors into preinitiated RNA chains. Synthesis with mercurated precursors was three- to fivefold slower than with nonmercurated nucleotides (12 to 20 pmol per 10(6) cells per h at 25 degrees C), and 50 to 70% of the RNA product bound specifically to sulfhydryl-agarose. The amount of mercurated CMP incorporated into RNA was measured by specific binding of [35S]cysteine to the mercury residues. More than 90% of the mercurated RNA hybridized to simian virus 40 DNA.

Duke Scholars

Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

January 1981

Volume

37

Issue

1

Start / End Page

511 / 517

Location

United States

Related Subject Headings

  • Virology
  • Transcription, Genetic
  • Subcellular Fractions
  • Simian virus 40
  • Ribonucleotides
  • RNA, Viral
  • Mercury
  • Kinetics
  • Kidney
  • Cytidine Triphosphate
 

Citation

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ICMJE
MLA
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Chikaraishi, D. M., & Danna, K. J. (1981). Simian virus 40 transcriptional complexes incorporate mercurated nucleotides into RNA in vitro. J Virol, 37(1), 511–517. https://doi.org/10.1128/JVI.37.1.511-517.1981
Chikaraishi, D. M., and K. J. Danna. “Simian virus 40 transcriptional complexes incorporate mercurated nucleotides into RNA in vitro.J Virol 37, no. 1 (January 1981): 511–17. https://doi.org/10.1128/JVI.37.1.511-517.1981.
Chikaraishi, D. M., and K. J. Danna. “Simian virus 40 transcriptional complexes incorporate mercurated nucleotides into RNA in vitro.J Virol, vol. 37, no. 1, Jan. 1981, pp. 511–17. Pubmed, doi:10.1128/JVI.37.1.511-517.1981.

Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

January 1981

Volume

37

Issue

1

Start / End Page

511 / 517

Location

United States

Related Subject Headings

  • Virology
  • Transcription, Genetic
  • Subcellular Fractions
  • Simian virus 40
  • Ribonucleotides
  • RNA, Viral
  • Mercury
  • Kinetics
  • Kidney
  • Cytidine Triphosphate