Elastin-like polypeptides as a purification tag for recombinant proteins.

Published

Journal Article

This unit presents a recombinant protein purification method that employs an elastin-like polypeptide (ELP) as a purification tag. ELPs undergo a sharp and reversible phase transition when heated above their lower critical solution temperature. ELPs retain this behavior when they are fused to a protein, and thereby provide a simple method to isolate a recombinant ELP fusion protein from cell contaminants by cycling the solution through the insoluble and soluble phase of the ELP fusion protein using a procedure that is termed Inverse Transition Cycling. This method does not require the use of chromatography, so it is cost-effective, easy to scale up, and easy to multiplex.

Full Text

Duke Authors

Cited Authors

  • Hassouneh, W; Christensen, T; Chilkoti, A

Published Date

  • August 2010

Published In

Volume / Issue

  • Chapter 6 /

Start / End Page

  • Unit - 6.11

PubMed ID

  • 20814933

Pubmed Central ID

  • 20814933

Electronic International Standard Serial Number (EISSN)

  • 1934-3663

International Standard Serial Number (ISSN)

  • 1934-3655

Digital Object Identifier (DOI)

  • 10.1002/0471140864.ps0611s61

Language

  • eng