Founder's Award, Society for Biomaterials. Sixth World Biomaterials Congress 2000, Kamuela, HI,May 15-20, 2000. Really smart bioconjugates of smart polymers and receptor proteins.

Journal Article (Review;Journal Article)

Over the past 18 years we have been deeply involved with the synthesis and applications of stimuli-responsive polymer systems, especially polymer-biomolecule conjugates. This article summarizes our work with one of these conjugate systems, specifically polymer-protein conjugates. We include conjugates prepared by random polymer conjugation to lysine amino groups, and also those prepared by site-specific conjugation of the polymer to specific amino acid sites that are genetically engineered into the known amino acid sequence of the protein. We describe the preparation and properties of thermally sensitive random conjugates to enzymes and several affinity recognition proteins. We have also prepared site-specific conjugates to streptavidin with temperature-sensitive polymers, pH-sensitive polymers, and light-sensitive polymers. The preparation of these conjugates and their many fascinating applications are reviewed in this article.

Full Text

Duke Authors

Cited Authors

  • Hoffman, AS; Stayton, PS; Bulmus, V; Chen, G; Chen, J; Cheung, C; Chilkoti, A; Ding, Z; Dong, L; Fong, R; Lackey, CA; Long, CJ; Miura, M; Morris, JE; Murthy, N; Nabeshima, Y; Park, TG; Press, OW; Shimoboji, T; Shoemaker, S; Yang, HJ; Monji, N; Nowinski, RC; Cole, CA; Priest, JH; Harris, JM; Nakamae, K; Nishino, T; Miyata, T

Published Date

  • December 2000

Published In

Volume / Issue

  • 52 / 4

Start / End Page

  • 577 - 586

PubMed ID

  • 11033539

Electronic International Standard Serial Number (EISSN)

  • 1097-4636

International Standard Serial Number (ISSN)

  • 0021-9304

Digital Object Identifier (DOI)

  • 10.1002/1097-4636(20001215)52:4<577::aid-jbm1>;2-5


  • eng