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Pancreatic secretory trypsin inhibitor I reduces the severity of chronic pancreatitis in mice overexpressing interleukin-1β in the pancreas.

Publication ,  Journal Article
Romac, JM-J; Shahid, RA; Choi, SS; Karaca, GF; Westphalen, CB; Wang, TC; Liddle, RA
Published in: Am J Physiol Gastrointest Liver Physiol
March 1, 2012

IL-1β is believed to play a pathogenic role in the development of pancreatitis. Expression of human IL-1β in pancreatic acinar cells produces chronic pancreatitis, characterized by extensive intrapancreatic inflammation, atrophy, and fibrosis. To determine if activation of trypsinogen is important in the pathogenesis of chronic pancreatitis in this model, we crossed IL-1β transgenic [Tg(IL1β)] mice with mice expressing a trypsin inhibitor that is normally produced in rat pancreatic acinar cells [pancreatic secretory trypsin inhibitor (PTSI) I]. We previously demonstrated that transgenic expression of PSTI-I [Tg(Psti1)] increased pancreatic trypsin inhibitor activity by 190%. Tg(IL1β) mice were found to have marked pancreatic inflammation, characterized by histological changes, including acinar cell loss, inflammatory cell infiltration, and fibrosis, as well as elevated myeloperoxidase activity and elevated pancreatic trypsin activity, as early as 6 wk of age. In contrast to Tg(IL1β) mice, pancreatitis was significantly less severe in dual-transgenic [Tg(IL1β)-Tg(Psti1)] mice expressing IL-1β and PSTI-I in pancreatic acinar cells. These findings indicate that overexpression of PSTI-I reduces the severity of pancreatitis and that pancreatic trypsin activity contributes to the pathogenesis of an inflammatory model of chronic pancreatitis.

Duke Scholars

Published In

Am J Physiol Gastrointest Liver Physiol

DOI

EISSN

1522-1547

Publication Date

March 1, 2012

Volume

302

Issue

5

Start / End Page

G535 / G541

Location

United States

Related Subject Headings

  • Trypsin Inhibitors
  • Trypsin Inhibitor, Kazal Pancreatic
  • Trypsin
  • Rats
  • Pancreatitis, Chronic
  • Pancreatitis
  • Pancreas
  • Mice
  • Male
  • Interleukin-1beta
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Romac, J.-J., Shahid, R. A., Choi, S. S., Karaca, G. F., Westphalen, C. B., Wang, T. C., & Liddle, R. A. (2012). Pancreatic secretory trypsin inhibitor I reduces the severity of chronic pancreatitis in mice overexpressing interleukin-1β in the pancreas. Am J Physiol Gastrointest Liver Physiol, 302(5), G535–G541. https://doi.org/10.1152/ajpgi.00287.2011
Romac, Joelle M-J, Rafiq A. Shahid, Steve S. Choi, Gamze F. Karaca, Christoph B. Westphalen, Timothy C. Wang, and Rodger A. Liddle. “Pancreatic secretory trypsin inhibitor I reduces the severity of chronic pancreatitis in mice overexpressing interleukin-1β in the pancreas.Am J Physiol Gastrointest Liver Physiol 302, no. 5 (March 1, 2012): G535–41. https://doi.org/10.1152/ajpgi.00287.2011.
Romac JM-J, Shahid RA, Choi SS, Karaca GF, Westphalen CB, Wang TC, et al. Pancreatic secretory trypsin inhibitor I reduces the severity of chronic pancreatitis in mice overexpressing interleukin-1β in the pancreas. Am J Physiol Gastrointest Liver Physiol. 2012 Mar 1;302(5):G535–41.
Romac, Joelle M. J., et al. “Pancreatic secretory trypsin inhibitor I reduces the severity of chronic pancreatitis in mice overexpressing interleukin-1β in the pancreas.Am J Physiol Gastrointest Liver Physiol, vol. 302, no. 5, Mar. 2012, pp. G535–41. Pubmed, doi:10.1152/ajpgi.00287.2011.
Romac JM-J, Shahid RA, Choi SS, Karaca GF, Westphalen CB, Wang TC, Liddle RA. Pancreatic secretory trypsin inhibitor I reduces the severity of chronic pancreatitis in mice overexpressing interleukin-1β in the pancreas. Am J Physiol Gastrointest Liver Physiol. 2012 Mar 1;302(5):G535–G541.

Published In

Am J Physiol Gastrointest Liver Physiol

DOI

EISSN

1522-1547

Publication Date

March 1, 2012

Volume

302

Issue

5

Start / End Page

G535 / G541

Location

United States

Related Subject Headings

  • Trypsin Inhibitors
  • Trypsin Inhibitor, Kazal Pancreatic
  • Trypsin
  • Rats
  • Pancreatitis, Chronic
  • Pancreatitis
  • Pancreas
  • Mice
  • Male
  • Interleukin-1beta