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Inverse correlation of maturity and antibacterial activity in human dendritic cells.

Publication ,  Journal Article
Buettner, M; Meinken, C; Bastian, M; Bhat, R; Stössel, E; Faller, G; Cianciolo, G; Ficker, J; Wagner, M; Röllinghoff, M; Stenger, S
Published in: J Immunol
April 1, 2005

Dendritic cells (DCs) are a key part of host defense against microbial pathogens, being part of the innate immune system, but also instructing the adaptive T cell response. This study was designed to evaluate whether human DCs directly contribute to innate immunity by killing intracellular bacteria, using tuberculosis as a model. DCs were detected in bronchoalveolar lavage samples indicating that DCs are available for immediate interaction with Mycobacterium tuberculosis (M. Tb) after inhalation of the pathogen. The phenotype of DC in bronchoalveolar lavage closely resembles monocyte-derived immature DC (iDC) according to the expression of CD1a, CD83, and CCR7. The antimicrobial activity of iDC against intracellular M. Tb inversely correlated with TNF-alpha-release and was enhanced by treatment with anti-TNF-alpha Abs. Differentiation of iDC into mature DC by addition of TNF-alpha or activation via Toll-like receptors further reduced killing of M. Tb. The antibacterial activity against intracellular M. Tb of all DCs was significantly lower than alveolar macrophages. Therefore, the maintenance of a pool of DCs at the site of disease activity in tuberculosis, and the maturation of these DC by TNF-alpha provides a mechanism by which M. Tb escapes the innate immune system.

Duke Scholars

Published In

J Immunol

DOI

ISSN

0022-1767

Publication Date

April 1, 2005

Volume

174

Issue

7

Start / End Page

4203 / 4209

Location

United States

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Mycobacterium tuberculosis
  • Macrophages, Alveolar
  • Immunophenotyping
  • Immunology
  • Immunity, Innate
  • Humans
  • Dendritic Cells
  • Cells, Cultured
  • Cell Differentiation
 

Citation

APA
Chicago
ICMJE
MLA
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Buettner, M., Meinken, C., Bastian, M., Bhat, R., Stössel, E., Faller, G., … Stenger, S. (2005). Inverse correlation of maturity and antibacterial activity in human dendritic cells. J Immunol, 174(7), 4203–4209. https://doi.org/10.4049/jimmunol.174.7.4203
Buettner, Maike, Christoph Meinken, Max Bastian, Rauf Bhat, Elmar Stössel, Gerhard Faller, George Cianciolo, et al. “Inverse correlation of maturity and antibacterial activity in human dendritic cells.J Immunol 174, no. 7 (April 1, 2005): 4203–9. https://doi.org/10.4049/jimmunol.174.7.4203.
Buettner M, Meinken C, Bastian M, Bhat R, Stössel E, Faller G, et al. Inverse correlation of maturity and antibacterial activity in human dendritic cells. J Immunol. 2005 Apr 1;174(7):4203–9.
Buettner, Maike, et al. “Inverse correlation of maturity and antibacterial activity in human dendritic cells.J Immunol, vol. 174, no. 7, Apr. 2005, pp. 4203–09. Pubmed, doi:10.4049/jimmunol.174.7.4203.
Buettner M, Meinken C, Bastian M, Bhat R, Stössel E, Faller G, Cianciolo G, Ficker J, Wagner M, Röllinghoff M, Stenger S. Inverse correlation of maturity and antibacterial activity in human dendritic cells. J Immunol. 2005 Apr 1;174(7):4203–4209.

Published In

J Immunol

DOI

ISSN

0022-1767

Publication Date

April 1, 2005

Volume

174

Issue

7

Start / End Page

4203 / 4209

Location

United States

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Mycobacterium tuberculosis
  • Macrophages, Alveolar
  • Immunophenotyping
  • Immunology
  • Immunity, Innate
  • Humans
  • Dendritic Cells
  • Cells, Cultured
  • Cell Differentiation