Inverse correlation of maturity and antibacterial activity in human dendritic cells.

Journal Article (Journal Article)

Dendritic cells (DCs) are a key part of host defense against microbial pathogens, being part of the innate immune system, but also instructing the adaptive T cell response. This study was designed to evaluate whether human DCs directly contribute to innate immunity by killing intracellular bacteria, using tuberculosis as a model. DCs were detected in bronchoalveolar lavage samples indicating that DCs are available for immediate interaction with Mycobacterium tuberculosis (M. Tb) after inhalation of the pathogen. The phenotype of DC in bronchoalveolar lavage closely resembles monocyte-derived immature DC (iDC) according to the expression of CD1a, CD83, and CCR7. The antimicrobial activity of iDC against intracellular M. Tb inversely correlated with TNF-alpha-release and was enhanced by treatment with anti-TNF-alpha Abs. Differentiation of iDC into mature DC by addition of TNF-alpha or activation via Toll-like receptors further reduced killing of M. Tb. The antibacterial activity against intracellular M. Tb of all DCs was significantly lower than alveolar macrophages. Therefore, the maintenance of a pool of DCs at the site of disease activity in tuberculosis, and the maturation of these DC by TNF-alpha provides a mechanism by which M. Tb escapes the innate immune system.

Full Text

Duke Authors

Cited Authors

  • Buettner, M; Meinken, C; Bastian, M; Bhat, R; Stössel, E; Faller, G; Cianciolo, G; Ficker, J; Wagner, M; Röllinghoff, M; Stenger, S

Published Date

  • April 1, 2005

Published In

Volume / Issue

  • 174 / 7

Start / End Page

  • 4203 - 4209

PubMed ID

  • 15778382

Pubmed Central ID

  • 15778382

International Standard Serial Number (ISSN)

  • 0022-1767

Digital Object Identifier (DOI)

  • 10.4049/jimmunol.174.7.4203


  • eng

Conference Location

  • United States