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Restriction of Legionella pneumophila growth in macrophages requires the concerted action of cytokine and Naip5/Ipaf signalling pathways.

Publication ,  Journal Article
Coers, J; Vance, RE; Fontana, MF; Dietrich, WF
Published in: Cell Microbiol
October 2007

Macrophages from the C57BL/6 (B6) mouse strain restrict intracellular growth of Legionella pneumophila, whereas A/J macrophages are highly permissive. The mechanism by which B6 macrophages restrict Legionella growth remains poorly understood, but is known to require the cytosolic microbe sensors Naip5 (Birc1e) and Ipaf. We hypothesized that Naip5 and Ipaf may act in partnership with other antimicrobial signalling pathways in macrophages. Indeed, we found that macrophages lacking either tumour necrosis factor (TNF)-alpha or type I interferon (IFN) signalling are permissive for growth of L. pneumophila, even in the presence of functional Naip5 and Ipaf alleles. Similarly, macrophages lacking Naip5 and/or Ipaf signalling were permissive even though we found that Naip5 or Ipaf were not required for induction of TNF-alpha and type I IFN. Therefore, our data suggest that the mechanism by which B6 macrophages restrict intracellular replication of L. pneumophila is more complex than previously appreciated, and involves the concerted action of cytokine and intracellular microbe sensor signalling pathways.

Duke Scholars

Published In

Cell Microbiol

DOI

ISSN

1462-5814

Publication Date

October 2007

Volume

9

Issue

10

Start / End Page

2344 / 2357

Location

India

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Signal Transduction
  • Neuronal Apoptosis-Inhibitory Protein
  • Microbiology
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Macrophages
  • Luminescent Measurements
  • Legionella pneumophila
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Coers, J., Vance, R. E., Fontana, M. F., & Dietrich, W. F. (2007). Restriction of Legionella pneumophila growth in macrophages requires the concerted action of cytokine and Naip5/Ipaf signalling pathways. Cell Microbiol, 9(10), 2344–2357. https://doi.org/10.1111/j.1462-5822.2007.00963.x
Coers, Jörn, Russell E. Vance, Mary F. Fontana, and William F. Dietrich. “Restriction of Legionella pneumophila growth in macrophages requires the concerted action of cytokine and Naip5/Ipaf signalling pathways.Cell Microbiol 9, no. 10 (October 2007): 2344–57. https://doi.org/10.1111/j.1462-5822.2007.00963.x.
Coers, Jörn, et al. “Restriction of Legionella pneumophila growth in macrophages requires the concerted action of cytokine and Naip5/Ipaf signalling pathways.Cell Microbiol, vol. 9, no. 10, Oct. 2007, pp. 2344–57. Pubmed, doi:10.1111/j.1462-5822.2007.00963.x.
Journal cover image

Published In

Cell Microbiol

DOI

ISSN

1462-5814

Publication Date

October 2007

Volume

9

Issue

10

Start / End Page

2344 / 2357

Location

India

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Signal Transduction
  • Neuronal Apoptosis-Inhibitory Protein
  • Microbiology
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Macrophages
  • Luminescent Measurements
  • Legionella pneumophila