Comparison of molecular markers in a cohort of patients with chronic myeloproliferative disorders.

Journal Article

Decreased expression of c-MPL protein in platelets, increased expression of polycythemia rubra vera 1 (PRV-1) and nuclear factor I-B (NFIB) mRNA in granulocytes, and loss of heterozygosity on chromosome 9p (9pLOH) were described as molecular markers for myeloproliferative disorders (MPDs). To assess whether these markers are clustered in subgroups of MPDs or represent independent phenotypic variations, we simultaneously determined their status in a cohort of MPD patients. Growth of erythropoietin-independent colonies (EECs) was measured for comparison. We observed concordance between EECs and PRV-1 in MPD patients across all diagnostic subclasses, but our results indicate that EECs remain the most reliable auxiliary test for polycythemia vera (PV). In contrast, c-MPL, NFIB, and 9pLOH constitute independent variations. Interestingly, decreased c-MPL and elevated PRV-1 also were observed in patients with hereditary thrombocythemia (HT) who carry a mutation in the thrombopoietin (TPO) gene. Thus, altered c-MPL and PRV-1 expression also can arise through a molecular mechanism different from sporadic MPD.

Full Text

Duke Authors

Cited Authors

  • Kralovics, R; Buser, AS; Teo, S-S; Coers, J; Tichelli, A; van der Maas, APC; Skoda, RC

Published Date

  • September 1, 2003

Published In

Volume / Issue

  • 102 / 5

Start / End Page

  • 1869 - 1871

PubMed ID

  • 12730106

International Standard Serial Number (ISSN)

  • 0006-4971

Digital Object Identifier (DOI)

  • 10.1182/blood-2003-03-0744

Language

  • eng

Conference Location

  • United States