Differential regulation of renin and Cox-2 expression in the renal cortex of C57Bl/6 mice.

Published

Journal Article

Based on the controversy about the relevance of cyclooxygenase-2 (Cox-2)-derived prostanoids from the macula densa for the control of the renin system, this study aimed to determine the interrelation between Cox-2 and renin expression in the mouse kidney. In control mice renin mRNA was readily detectable whilst renocortical Cox-2 mRNA abundance was at the detection limit of the RNase protection assay and no specific signals for Cox-2 were obtained by in situ hybridization or Western blot analysis. Experimental maneuvers such as low-salt diet, treatment with loop diuretics or angiotensin I converting enzyme inhibitors clearly increased renin mRNA abundance up to sevenfold, but under none of these conditions renocortical Cox-2 mRNA levels were significantly changed. Moreover, the strong stimulation of renin expression by angiotensin I-converting enzyme inhibition was not changed by the cyclooxygenase inhibitor ibuprofen, which in turn clearly lowered tissue prostanoid content. Our data suggest a marked divergence of renin and Cox-2 expression in the kidney cortex of C57Bl/6 mice with no clear evidence for a role of Cox-2-derived prostanoids from the macula densa in the regulation of renin expression.

Full Text

Duke Authors

Cited Authors

  • Wagner, C; Vitzthum, H; Castrop, H; Schumacher, K; Bucher, M; Albertin, S; Coffman, TM; Arendshorst, WJ; Kurtz, A

Published Date

  • November 2003

Published In

Volume / Issue

  • 447 / 2

Start / End Page

  • 214 - 222

PubMed ID

  • 14504926

Pubmed Central ID

  • 14504926

International Standard Serial Number (ISSN)

  • 0031-6768

Digital Object Identifier (DOI)

  • 10.1007/s00424-003-1157-1

Language

  • eng

Conference Location

  • Germany